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PMID: 3665875 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Activity of two different silencer elements of the chicken lysozyme gene can be compensated by enhancer elements.

The EMBO journal ·Vol. 6 ·No. 8 ·1987-08-00 ·Pages 2297-303

Baniahmad A, Muller M, Steiner C, Renkawitz R

Abstract

The chicken lysozyme gene is constitutively expressed in macrophages. Transfection of recombinant genes containing different portions of the lysozyme 5' upstream region revealed the existence of two negative transcriptional elements within 1 kb upstream of the start sites. Both elements placed upstream or downstream of a heterologous promoter-gene unit repress transcription independent of their orientation and are therefore called silencer elements, although their repressing activities 3' of the gene are reduced. One silencer (N-1.0 kb) at position -1 kb consists of the central region of the chicken middle repetitive sequence element CR1 and can be divided into two functional domains. N-1.0 kb is active in all cell types tested. The other silencer (N-0.25 kb) at position -0.25 kb shows reduced activity in primary macrophages. Despite their different specificities, the activity of both silencer elements can be influenced similarly. An inverse linear relationship between the transcriptional activity of the tested constructs and the potential inhibition by the silencer elements was found: weak transcription units can be strongly repressed, whereas strong transcription units can be only weakly repressed. Such a mechanism may help to turn off completely a particular gene in situations or tissues where strong positive regulators are inactive.

MeSH Terms
Animals Base Sequence Chickens Chromosome Deletion Enhancer Elements, Genetic Genes Genes, Regulator Macrophages/enzymology Muramidase/genetics Mutation Plasmids
Chemicals
Muramidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Baniahmad A
Max-Planck-Institut für Biochemie, Genzentrum, Martinsried, FRG.
Muller M
Steiner C
Renkawitz R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-08-00
Pages
2297-303
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553632
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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