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PMID: 3399388 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Overlapping open reading frames revealed by complete nucleotide sequencing of turnip yellow mosaic virus genomic RNA.

Nucleic acids research ·Vol. 16 ·No. 13 ·1988-07-11 ·Pages 6157-73

Morch MD, Boyer JC, Haenni AL

Abstract

The complete nucleotide sequence of turnip yellow mosaic virus (TYMV) genomic RNA has been determined on a set of overlapping cDNA clones using a sequential sequencing strategy. The RNA is 6318 nucleotides long, excluding the cap structure. The genome organization deduced from the sequence confirms previous results of in vitro translation. A novel open reading frame (ORF) putatively encoding a Pro-rich and very basic 69K (K = kilodalton) protein is detected at the 5' end of the genome. It is initiated at the first AUG codon on the RNA and overlaps the major ORF that encodes the non structural 206K (previously referred to as 195K) protein of TYMV; its function is unknown. Several amino acid consensus sequences already described among plant and animal viruses are also found in the TYMV-encoded polypeptides. A comparison with other viruses whose RNA sequence is known leads to the conclusion that TYMV belongs to the "Sindbis-like" supergroup of viruses and could be related to Semliki forest virus.

MeSH Terms
Amino Acid Sequence Base Sequence Codon DNA/analysis Molecular Sequence Data Plant Viruses/genetics Polymorphism, Genetic Protein Biosynthesis RNA, Viral/analysis
Chemicals
Codon RNA, Viral DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Morch M D
Institut Jacques Monod, CNRS-Université Paris VII, Paris, France.
Boyer J C
Haenni A L
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.13.6157
Published
1988-07-11
Pages
6157-73
Language
English
Region
England
NLM ID
0411011
PMCID
PMC336854
Subset
IM
Databases
GENBANK
X07441
External Links
PubMed source DOI: 10.1093/nar/16.13.6157 PMC Full Text View journal details
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