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PMID: 16453487 Published · ppublish English Journal Article

The nucleotide sequence of cowpea mosaic virus B RNA.

The EMBO journal ·Vol. 2 ·No. 12 ·1983-00-00 ·Pages 2253-8

Lomonossoff GP, Shanks M

Abstract

The complete sequence of the bottom component RNA (B RNA) of cowpea mosaic virus (CPMV) has been determined. Restriction enzyme fragments of double-stranded cDNA were cloned in M13 and the sequence of the inserts was determined by a combination of enzymatic and chemical sequencing techniques. Additional sequence information was obtained by primed synthesis on first strand cDNA. The complete sequence deduced is 5889 nucleotides long excluding the 3' poly(A), and contains an open reading frame sufficient to code for a polypeptide of mol. wt. 207 760. The coding region is flanked by a 5' leader sequence of 206 nucleotides and a 3' non-coding region of 82 residues which does not contain a polyadenylation signal.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lomonossoff G P
Department of Virus Research, John Innes Institute, Colney Lane, Norwich NR4 7UH, UK.
Shanks M
References (42)
42 references, click to expand
  1. Molecular mechanisms of nucleotide-sequence rearrangements in cDNA clones of human fibroblast interferon mRNA.
    Gene. 1981 Nov;15(2-3):215-23 PMID: 6170541
  2. Translation of cowpea mosaic virus RNA in a cell-free extract from wheat germ.
    FEBS Lett. 1977 May 15;77(2):265-9 PMID: 862928
  3. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  4. Homology between human bladder carcinoma oncogene product and mitochondrial ATP-synthase.
    Nature. 1983 Jan 20;301(5897):262-4 PMID: 6296696
  5. Complete nucleotide sequence of alfalfa mosaic virus RNA 2.
    Nucleic Acids Res. 1983 May 25;11(10):3019-25 PMID: 6304618
  6. Nucleotide-sequence heterogeneity and sequence rearrangements in influenza virus cDNA.
    Gene. 1981 Nov;15(2-3):207-14 PMID: 6895362
  7. Cucumber mosaic virus RNA 3. Determination of the nucleotide sequence provides the amino acid sequences of protein 3A and viral coat protein.
    Eur J Biochem. 1982 Aug;126(2):217-26 PMID: 6927855
  8. Nucleotide sequence of tobacco mosaic virus RNA.
    Proc Natl Acad Sci U S A. 1982 Oct;79(19):5818-22 PMID: 6964389
  9. Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.
    Nucleic Acids Res. 1981 Apr 10;9(7):1691-706 PMID: 7232224
  10. Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes.
    Nucleic Acids Res. 1981 Oct 24;9(20):5233-52 PMID: 7301588
  11. A new computer method for the storage and manipulation of DNA gel reading data.
    Nucleic Acids Res. 1980 Aug 25;8(16):3673-94 PMID: 7433103
  12. Expression of Middle-Component RNA of Cowpea Mosaic Virus: In Vitro Generation of a Precursor to Both Capsid Proteins by a Bottom-Component RNA-Encoded Protease from Infected Cells.
    J Virol. 1982 Jan;41(1):8-17 PMID: 16789216
  13. Complementation and pseudorecombination between ribonucleic acids from two natural isolates of cowpea mosaic virus (severe subgroup).
    Virology. 1978 Mar;85(1):75-83 PMID: 77077
  14. Location of the gene specifying the smaller protein of the cowpea mosaic virus capsid.
    Virology. 1977 Jun 15;79(2):259-66 PMID: 867824
  15. Dictyostelium 17S, 25S, and 5S rDNAs lie within a 38,000 base pair repeated unit.
    Cell. 1976 Nov;9(3):431-8 PMID: 1033038
  16. Infectivity of a mixture of cowpea mosaic virus ribonucleoprotein components.
    Virology. 1967 Jun;32(2):306-20 PMID: 4960987
  17. Nucleotide sequence of Moloney murine leukaemia virus.
    Nature. 1981 Oct 15-21;293(5833):543-8 PMID: 6169994
  18. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  19. A method for sequencing single-stranded cloned DNA in both directions.
    Biosci Rep. 1981 Mar;1(3):243-52 PMID: 6271278
  20. Cowpea mosaic virus RNAs have neither m7GpppN ... nor mono-, di- or triphosphates at their 5' ends.
    Cell. 1977 May;11(1):73-82 PMID: 872220
  21. Polyadenylate sequences in the ribonucleic acids of cowpea mosaic virus.
    Virology. 1973 Nov;56(1):198-206 PMID: 4745624
  22. Purification and properties of the components of cowpea mosaic virus.
    Virology. 1967 Apr;31(4):633-42 PMID: 6022691
  23. Sequence of 1060 3'-terminal nucleotides of poliovirus RNA as determined by a modification of the dideoxynucleotide method.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3196-200 PMID: 6158042
  24. Complete nucleotide sequence of alfalfa mosaic virus RNA 1.
    Nucleic Acids Res. 1983 Mar 11;11(5):1253-65 PMID: 6298738
  25. Primary structure and gene organization of the middle-component RNA of cowpea mosaic virus.
    EMBO J. 1983;2(6):941-6 PMID: 6641721
  26. Usage of the three termination codons: compilation and analysis of the known eukaryotic and prokaryotic translation termination sequences.
    Mol Gen Genet. 1981;182(3):430-9 PMID: 6946272
  27. tRNA punctuation model of RNA processing in human mitochondria.
    Nature. 1981 Apr 9;290(5806):470-4 PMID: 7219536
  28. Comparison of in vivo and in vitro translation of cowpea mosaic virus RNAs.
    Biochem Biophys Res Commun. 1981 Mar 16;99(1):89-94 PMID: 7236272
  29. Expression of bottom component RNA of cowpea mosaic virus in cowpea protoplasts.
    J Virol. 1980 Nov;36(2):366-73 PMID: 16789203
  30. Orientation of the cleavage map of the 200-kilodalton polypeptide encoded by the bottom-component RNA of cowpea mosaic virus.
    J Virol. 1983 May;46(2):614-9 PMID: 16789243
  31. Sequence homology adjacent to the 3' terminal poly(A) of cowpea mosaic virus RNAs.
    Nucleic Acids Res. 1979 Sep 25;7(2):493-500 PMID: 91160
  32. Nucleotide sequences adjacent to the proteins covalently linked to the cowpea mosaic virus genome. Sequence determination after labelling in vitro using RNA ligase.
    Eur J Biochem. 1979 Nov 1;101(1):45-9 PMID: 116852
  33. Protein bound to the genome RNAs of cowpea mosaic virus.
    Eur J Biochem. 1978 Dec 1;92(1):45-51 PMID: 215411
  34. Determination of the length distribution of poly(A) at the 3' terminus of the virion RNAs of EMC virus, poliovirus, rhinovirus, RAV-61 and CPMV and of mouse globin mRNA.
    Nucleic Acids Res. 1979 Nov 10;7(5):1195-204 PMID: 229465
  35. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  36. Synthesis and proteolytic processing of cowpea mosaic virus proteins in reticulocyte lysates.
    Virology. 1979 Jul 30;96(2):463-77 PMID: 462814
  37. The 5' end group of tobacco mosaic virus RNA is m7G5' ppp5' Gp.
    Nucleic Acids Res. 1975 Jul;2(7):1189-201 PMID: 168558
  38. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  39. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  40. Leaky UAG termination codon in tobacco mosaic virus RNA.
    Nature. 1978 Mar 30;272(5652):469-71 PMID: 634374
  41. A protein linked to the 5' termini of both RNA components of the cowpea mosaic virus genome.
    Nucleic Acids Res. 1978 Dec;5(12):4505-22 PMID: 745988
  42. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1983-00-00
Pages
2253-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC555442
External Links
PubMed source PMC Full Text View journal details
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