A protein linked to the 5' termini of both RNA components of the cowpea mosaic virus genome.

Nucleic acids research ·Vol. 5 ·No. 12 ·1978-12-00 ·Pages 4505-22

Stanley J, Rottier P, Davies JW, Zabel P, Van Kammen A

Abstract

Evidence is presented for the presence of a protein covalently bound to the 5' termini of both M and B RNA components of CPMV. The protein is found to be linked in both cases to the 5' phosphate of the dinucleotide pUpAp, derived by ribonuclease digestion of the RNA. The intact protein is not required for infectivity or for in vitro translation of the RNA in cell-free extracts.

MeSH Terms

Mosaic Viruses/metabolism Peptide Hydrolases Plants/metabolism Protein Binding Protein Biosynthesis RNA, Viral/isolation & purification,metabolism Triticum/metabolism Viral Proteins/isolation & purification,metabolism Virus Replication

Chemicals

RNA, Viral Viral Proteins Peptide Hydrolases

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Stanley J

Rottier P

Davies J W

Zabel P

Van Kammen A

References (18)

18 references, click to expand

Cowpea mosaic virus RNAs have neither m7GpppN ... nor mono-, di- or triphosphates at their 5' ends.
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Encephalomyocarditis virus RNA. III. Presence of a genome-associated protein.
J Virol. 1978 Jan;25(1):413-5 PMID: 202751
Protein is linked to the 5' end of poliovirus RNA by a phosphodiester linkage to tyrosine.
J Biol Chem. 1978 Aug 10;253(15):5263-6 PMID: 209034
The 5'-terminal structures of poliovirion RNA and poliovirus mRNA differ only in the genome-linked protein VPg.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5345-9 PMID: 202952
Protein covalently linked to foot-and-mouth disease virus RNA.
Nature. 1977 Aug 18;268(5621):648-50 PMID: 197422
The location of the polio genome protein in viral RNAs and its implication for RNA synthesis.
Nature. 1977 Jul 21;268(5617):208-13 PMID: 196204
Covalent linkage of a protein to a defined nucleotide sequence at the 5'-terminus of virion and replicative intermediate RNAs of poliovirus.
Proc Natl Acad Sci U S A. 1977 Mar;74(3):961-5 PMID: 191841
A protein covalently linked to poliovirus genome RNA.
Proc Natl Acad Sci U S A. 1977 Jan;74(1):59-63 PMID: 189316
Translation of cowpea mosaic virus RNA in a cell-free extract from wheat germ.
FEBS Lett. 1977 May 15;77(2):265-9 PMID: 862928
Ribonucleic acid isolated by cesium chloride centrifugation.
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Plant viruses with a multipartite genome.
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Gel electrophoresis of RNA under denaturing conditions.
Biochim Biophys Acta. 1973 Oct 26;324(3):320-33 PMID: 4202717
The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent.
Biochem J. 1973 Jul;133(3):529-39 PMID: 4733239
Polyadenylate sequences in the ribonucleic acids of cowpea mosaic virus.
Virology. 1973 Nov;56(1):198-206 PMID: 4745624
Suppression of RNA precipitation during Cs2SO4 density gradient centrifugation.
Biochem Biophys Res Commun. 1966 Jun 13;23(5):612-8 PMID: 4960466
A two-dimensional fractionation procedure for radioactive nucleotides.
J Mol Biol. 1965 Sep;13(2):373-98 PMID: 5325727
Genetic analysis of cowpea mosaic virus mutants by supplementation and reassortment tests.
Virology. 1976 Mar;70(1):151-63 PMID: 1258375
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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/5.12.4505

Published

1978-12-00

Pages

4505-22

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342769

Subset

External Links

PubMed source DOI: 10.1093/nar/5.12.4505 PMC Full Text View journal details

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A protein linked to the 5' termini of both RNA components of the cowpea mosaic virus genome.

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