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PMID: 3380092 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transformation of Tetrahymena thermophila with hypermethylated rRNA genes.

Molecular and cellular biology ·Vol. 8 ·No. 4 ·1988-04-00 ·Pages 1664-9

Karrer KM, Yao MC

Abstract

The extrachromosomal rRNA genes (rDNA) of Tetrahymena thermophila contain 0.4% N6-methyladenine. C3 strain rDNA was isolated, hypermethylated in vitro, and microinjected into B strain host cells. Clonal cell lines were established, and transformants were selected on the basis of resistance to paromomycin, conferred by the injected rDNA. The effects of methylation by three enzymes which methylate the sequence 5'-NAT-3', the dam, EcoRI, and ClaI methylases, were tested. Hypermethylation of the injected rDNA had no effect on transformation efficiency relative to mock-methylated controls. The injected C3 strain rDNA efficiently replaced host rDNA as the major constituent of the population of rDNA molecules. Hypermethylation of the injected DNA was not maintained through 20 to 25 cell generations.

MeSH Terms
Animals Cell Division Cell Line DNA, Ribosomal/genetics Genes Methylation RNA, Ribosomal/genetics Tetrahymena/genetics Transformation, Genetic
Chemicals
DNA, Ribosomal RNA, Ribosomal
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Karrer K M
Department of Biology, Brandeis University, Waltham, Massachusetts 02254.
Yao M C
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32 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.4.1664-1669.1988
Published
1988-04-00
Pages
1664-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363326
Subset
IM
Grants
NIGMS NIH HHS · GM26210 · United States
NIGMS NIH HHS · GM32989 · United States
NICHD NIH HHS · HD00547 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.4.1664-1669.1988 PMC Full Text View journal details
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