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PMID: 6950932 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sequence specificity of DNA adenine methylase in the protozoan Tetrahymena thermophila.

Journal of bacteriology ·Vol. 150 ·No. 2 ·1982-05-00 ·Pages 993-6

Bromberg S, Pratt K, Hattman S

Abstract

The sequence specificity of the Tetrahymena DNA-adenine methylase was determined by nearest-neighbor analyses of in vivo and in vitro methylated DNA. In vivo all four common bases were found to the 5' side of N6-methyladenine, but only thymidine was 3'. Homologous DNA already methylated in vivo and heterologous Micrococcus luteus DNA were methylated in vitro by a partially purified DNA-adenine methylase activity isolated from Tetrahymena macronuclei. The in vitro-methylated sequence differed from the in vivo sequence in that both thymidine and cytosine were 3' nearest neighbors of N6-methyladenine.

MeSH Terms

Adenine/analogs & derivatives Animals Base Sequence Cytosine DNA/metabolism DNA (Cytosine-5-)-Methyltransferases/metabolism DNA, Bacterial/metabolism Methyltransferases/metabolism Site-Specific DNA-Methyltransferase (Adenine-Specific) Substrate Specificity Tetrahymena/enzymology Thymidine

Chemicals

DNA, Bacterial Cytosine DNA Methyltransferases DNA (Cytosine-5-)-Methyltransferases Site-Specific DNA-Methyltransferase (Adenine-Specific) Adenine Thymidine 6-methyladenine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Bromberg S

Pratt K

Hattman S

References (14)

14 references, click to expand

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DNA modification mechanisms and gene activity during development.
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X inactivation, differentiation, and DNA methylation.
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Isolation of micro- and macronuclei of Tetrahymena pyriformis.
Methods Cell Biol. 1975;9(0):311-27 PMID: 805898
Selective silencing of eukaryotic DNA.
Science. 1975 Aug 8;189(4201):426-33 PMID: 1098145
Sequence specificity of the wild-type dam+) and mutant (damh) forms of bacteriophage T2 DNA adenine methylase.
J Mol Biol. 1978 Mar 5;119(3):361-76 PMID: 641992
Modified bases in the DNAs of unicellular eukaryotes: an examination of distributions and possible roles, with emphasis on hydroxymethyluracil in dinoflagellates.
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Comparative study of DNA methylation in three unicellular eucaryotes.
J Bacteriol. 1978 Sep;135(3):1156-7 PMID: 99431
Sequence specificity of DNA methylases from Bacillus amyloliquefaciens and Bacillus brevis.
J Mol Biol. 1978 Oct 5;124(4):701-11 PMID: 712853
Sequence specificity of the P1 modification methylase (M.Eco P1) and the DNA methylase (M.Eco dam) controlled by the Escherichia coli dam gene.
J Mol Biol. 1978 Dec 15;126(3):367-80 PMID: 370402
DNA methylation and gene function.
Science. 1980 Nov 7;210(4470):604-10 PMID: 6254144
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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.150.2.993-996.1982

Published

1982-05-00

Pages

993-6

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC216459

Subset

Grants

NIAID NIH HHS · AI-10864 · United States

NIGMS NIH HHS · GM-29227 · United States

External Links

PubMed source DOI: 10.1128/jb.150.2.993-996.1982 PMC Full Text View journal details

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Sequence specificity of DNA adenine methylase in the protozoan Tetrahymena thermophila.

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