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PMID: 3031472 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence structure and consistency of a developmentally regulated DNA deletion in Tetrahymena thermophila.

Molecular and cellular biology ·Vol. 7 ·No. 1 ·1987-01-00 ·Pages 435-43

Austerberry CF, Yao MC

Abstract

DNA deletion by site-specific chromosome breakage and rejoining occurs extensively during macronuclear development in the ciliate Tetrahymena thermophila. We have sequenced both the micronuclear (germ line) and rearranged macronuclear (somatic) forms of one region from which 1.1 kilobases of micronuclear DNA are reproducibly deleted during macronuclear development. The deletion junctions lie within a pair of 6-base-pair direct repeats. The termini of the deleted sequence are not inverted repeats. The precision of deletion at the nucleotide level was also characterized by hybridization with a synthetic oligonucleotide matching the determined macronuclear (rejoined) junction sequence. This deletion occurs in a remarkably sequence-specific manner. However, a very minor degree of variability in the macronuclear junction sequences was detected and was shown to be inherent in the mechanism of deletion itself. These results suggest that DNA deletion during macronuclear development in T. thermophila may constitute a novel type of DNA recombination and that it can create sequence heterogeneity on the order of a few base pairs at rejoining junctions.

MeSH Terms
Animals Base Sequence Chromosome Deletion Cloning, Molecular DNA/analysis DNA Restriction Enzymes Nucleic Acid Hybridization Tetrahymena/genetics,growth & development
Chemicals
DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Austerberry C F
Yao M C
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50 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.1.435-443.1987
Published
1987-01-00
Pages
435-43
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365086
Subset
IM
Grants
NIGMS NIH HHS · GM07067 · United States
NIGMS NIH HHS · GM26210 · United States
NICHD NIH HHS · HD00547 · United States
Databases
GENBANK
M16034, M17264
External Links
PubMed source DOI: 10.1128/mcb.7.1.435-443.1987 PMC Full Text View journal details
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