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PMID: 3982421 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Elimination of micronuclear specific DNA sequences early in anlagen development.

Molecular and cellular biology ·Vol. 5 ·No. 1 ·1985-01-00 ·Pages 93-8

Brunk CF, Conover RK

Abstract

After conjugation in Tetrahymena thermophila, the old macronuclei degenerate, and new macronuclei (anlagen) develop. During anlagen development a number of DNA sequences found in the micronuclear genome (micronuclear limited sequences) are eliminated from the anlagen. A cloned copy of a repetitive micronuclear limited sequence has been used to determine the developmental stage at which micronuclear limited sequences are eliminated. DNAs from anlagen of various developmental stages were examined by Southern analysis. It was found that micronuclear limited sequences are present in 4C anlagen and essentially absent in 8C and 16C anlagen. The precipitous loss of these sequences in the 8C anlagen rules out under-replication as the mechanism for the loss and suggests that these sequences are specifically degraded early during anlagen development.

MeSH Terms

Animals Base Sequence Cell Nucleus/physiology DNA/genetics Gene Expression Regulation Tetrahymena/genetics Time Factors

Chemicals

DNA

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Brunk C F

Conover R K

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28 references, click to expand

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Article Info

Journal

Molecular and cellular biology

Abbr.

Mol Cell Biol

ISSN

0270-7306

DOI

10.1128/mcb.5.1.93-98.1985

Published

1985-01-00

Pages

93-8

Language

English

Region

United States

NLM ID

8109087

PMCID

PMC366682

Subset

Grants

NCRR NIH HHS · 5-S07 RR07009-17 · United States

External Links

PubMed source DOI: 10.1128/mcb.5.1.93-98.1985 PMC Full Text View journal details

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Elimination of micronuclear specific DNA sequences early in anlagen development.

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