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PMID: 6473102 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Internal micronuclear DNA regions which include sequences homologous to macronuclear telomeres are deleted during development in Tetrahymena.

Nucleic acids research ·Vol. 12 ·No. 15 ·1984-08-10 ·Pages 6103-16

Yokoyama R, Yao MC

Abstract

C4A2 repeats are present in multiple clusters in both the macronucleus and micronucleus of Tetrahymena. Although the macronucleus is generated from the micronucleus after sexual conjugation, the repeats are telomeric sequences in the macronucleus but are internally located in the micronucleus (1). This study investigates the fate of the sequences adjacent to the micronuclear C4A2 repeats. Southern blot analyses of 21 C4A2-containing micronuclear clones show that extensive elimination of the adjacent sequences occurs during the formation of the macronucleus. Comparison of one C4A2-containing micronuclear clone with its derived macronuclear segment indicates that approximately 4.5 kb of DNA, which includes the C4A2 repeats and adjacent sequences on both sides is deleted from the macronucleus. The two regions adjoining the deletion are joined together to form a contiguous segment in the macronucleus. This excision of C4A2 repeats and surrounding sequences and the rejoining of the retained segments is probably the mechanism by which all or most of the other C4A2 adjacent sequences are eliminated.

MeSH Terms
Animals Base Sequence Cell Nucleus/ultrastructure Chromosome Deletion Chromosome Mapping Chromosomes/ultrastructure Gene Amplification Gene Expression Regulation Repetitive Sequences, Nucleic Acid Tetrahymena/genetics
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Yokoyama R
Yao M C
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30 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.15.6103
Published
1984-08-10
Pages
6103-16
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320060
Subset
IM
Grants
NIGMS NIH HHS · GM 26210 · United States
External Links
PubMed source DOI: 10.1093/nar/12.15.6103 PMC Full Text View journal details
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