Home LiteratureArticle Details
PMID: 2877440 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A family of DNA sequences is reproducibly rearranged in the somatic nucleus of Tetrahymena.

Nucleic acids research ·Vol. 14 ·No. 20 ·1986-10-24 ·Pages 8007-25

Allitto BA, Karrer KM

Abstract

A small family of DNA sequences is rearranged during the development of the somatic nucleus in Tetrahymena. The family is defined by 266 bp of highly conserved sequence which restriction mapping, hybridization and sequence analysis have shown is shared by a cloned micronuclear fragment and three sequences which constitute the macronuclear family. Genomic Southern hybridization experiments indicate there are five members of the family in micronuclear DNA. All of the family members are present in whole genome homozygotes and are therefore nonallelic. The three macronuclear sequences are all present in clonal cell lines and are reproducibly generated in every developing macronucleus. The rearrangement event begins 14 hours after conjugation is initiated and is nearly completed by 16 hours.

MeSH Terms
Animals Cell Differentiation Cell Nucleus/physiology Chromosome Mapping Gene Expression Regulation Genes Multigene Family Polymorphism, Restriction Fragment Length Recombination, Genetic Reproduction Tetrahymena/genetics,growth & development
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Allitto B A
Karrer K M
References (31)
31 references, click to expand
  1. Rearrangement of repeated DNA sequences during development of macronucleus in Tetrahymena thermophila.
    Nucleic Acids Res. 1982 Jul 24;10(14):4279-91 PMID: 6289270
  2. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.
    DNA. 1985 Apr;4(2):165-70 PMID: 3996185
  3. DNA elimination in Tetrahymena: a developmental process involving extensive breakage and rejoining of DNA at defined sites.
    Cell. 1984 Feb;36(2):433-40 PMID: 6319023
  4. Identification and purification of young macronuclear anlagen from conjugating cells of Tetrahymena thermophila.
    Dev Biol. 1982 Oct;93(2):519-33 PMID: 7141113
  5. Amplification of the rRNA genes in Tetrahymena.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 2:1293-6 PMID: 290442
  6. Reorganization of unique and repetitive sequences during nuclear development in Tetrahymena thermophila.
    Can J Biochem. 1982 Sep;60(9):847-53 PMID: 7172094
  7. Elimination of micronuclear specific DNA sequences early in anlagen development.
    Mol Cell Biol. 1985 Jan;5(1):93-8 PMID: 3982421
  8. Germ line-specific DNA sequences are present on all five micronuclear chromosomes in Tetrahymena thermophila.
    Mol Cell Biol. 1983 Nov;3(11):1909-19 PMID: 6656759
  9. Internal sequences are eliminated from genes during macronuclear development in the ciliated protozoan Oxytricha nova.
    Cell. 1984 Apr;36(4):1045-55 PMID: 6323015
  10. Developmental rearrangements associated with a single type of expressed alpha-tubulin gene in Tetrahymena.
    Cell. 1984 Feb;36(2):441-5 PMID: 6319024
  11. Nucleotide sequence of the rightward operator of phage lambda.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 PMID: 1055375
  12. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  13. Cytogenetics of genomic exclusion in Tetrahymena.
    Genetics. 1967 Apr;55(4):797-822 PMID: 6036954
  14. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  15. Cytochemical studies on the problem of macronuclear subnuclei in tetrahymena.
    Genetics. 1972 Feb;70(2):251-60 PMID: 17248560
  16. Numbers of 5S and tRNA genes in macro- and micronuclei of Tetrahymena pyriformis.
    Chromosoma. 1976 Mar 10;54(4):327-37 PMID: 815076
  17. Elimination of specific DNA sequences from the somatic nucleus of the ciliate Tetrahymena.
    J Cell Biol. 1982 Mar;92(3):783-9 PMID: 6282893
  18. Tetrahymena conjugation-induced genes: structure and organization in macro- and micronuclei.
    Nucleic Acids Res. 1986 Feb 11;14(3):1341-54 PMID: 3005971
  19. Reproducible and variable genomic rearrangements occur in the developing somatic nucleus of the ciliate Tetrahymena thermophila.
    Mol Cell Biol. 1985 Aug;5(8):2039-50 PMID: 3018547
  20. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  21. Induction and isolation of mutants in Tetrahymena.
    Methods Cell Biol. 1976;13:247-82 PMID: 817110
  22. Specific DNA rearrangements in synchronously developing nuclei of Tetrahymena.
    Proc Natl Acad Sci U S A. 1984 Dec;81(23):7383-7 PMID: 6095290
  23. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  24. Nullisomic Tetrahymena. II. a Set of Nullisomics Define the Germinal Chromosomes.
    Genetics. 1983 Jun;104(2):257-70 PMID: 17246135
  25. Isolation of micro- and macronuclei of Tetrahymena pyriformis.
    Methods Cell Biol. 1975;9(0):311-27 PMID: 805898
  26. Site-specific methylation of adenine in the nuclear genome of a eucaryote, Tetrahymena thermophila.
    Mol Cell Biol. 1986 Jul;6(7):2364-70 PMID: 3023930
  27. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis.
    Gene. 1983 Dec;26(1):101-6 PMID: 6323249
  28. Macronuclear DNA of Tetrahymena thermophila exists as defined subchromosomal-sized molecules.
    Nucleic Acids Res. 1985 Aug 26;13(16):5817-31 PMID: 4034396
  29. Three different macronuclear DNAs in Oxytricha fallax share a common sequence block.
    Mol Cell Biol. 1984 May;4(5):931-8 PMID: 6328276
  30. Reduction in the rate of DNA reassociation by sequence divergence.
    J Mol Biol. 1973 Dec 5;81(2):123-35 PMID: 4591183
  31. Conjugation in Tetrahymena thermophila. A temporal analysis of cytological stages.
    Exp Cell Res. 1982 Jul;140(1):227-36 PMID: 7106201
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.20.8007
Published
1986-10-24
Pages
8007-25
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311831
Subset
IM
Grants
NIGMS NIH HHS · GM 32989 · United States
NCRR NIH HHS · S07 RR07044 · United States
Databases
GENBANK
X04496, X04497, X04498
External Links
PubMed source DOI: 10.1093/nar/14.20.8007 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2