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PMID: 333453 Published · ppublish English Journal Article

Specialized transducing phage for the initiation factor 3 gene in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 9 ·1977-09-00 ·Pages 3970-4

Springer M, Graffe M, Hennecke H

Abstract

A previously isolated thermosensitive mutant [Springer, M., Graffe, M. & Grunberg-Manago, M. (6977) Mol. Gen. Genet. 151, 17-26] exhibits two defects in vitro, one in the initiation factor IF3 and the other in the L-phenylalanine: tRNA-Phe ligase (EC 6.1.1.20). Specialized lambda transducing phages that transduced this mutant to thermoresistance were selected. In vitro studies showed that the transductants had a normal IF3 activity. One of these transducing phages was shown to code for a protein synthesized under the control of Escherichia coli promoters, which has the same molecular weight as IF3. This protein crossreacts specifically with IF3 antisera and comigrates with pure IF3 in a two-dimensional gel system.

MeSH Terms
Bacterial Proteins/biosynthesis Coliphages/genetics DNA, Recombinant/metabolism Escherichia coli/genetics Genotype Kinetics Lysogeny Molecular Weight Peptide Initiation Factors Protein Biosynthesis Transduction, Genetic Viral Proteins/biosynthesis
Chemicals
Bacterial Proteins DNA, Recombinant Peptide Initiation Factors Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Springer M
Graffe M
Hennecke H
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.9.3970
Published
1977-09-00
Pages
3970-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431805
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.9.3970 PMC Full Text View journal details
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