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PMID: 325363 Published · ppublish English Journal Article

Characterization of an E. coli mutant with a thermolabile initiation factor IF3 activity.

Molecular & general genetics : MGG ·Vol. 151 ·No. 1 ·1977-02-28 ·Pages 17-26

Springer M, Graffe M, Grunberg-Manago M

Abstract

A thermosensitive E. coli mutant is described which has at least two defects in vitro: a thermolabile initiation factor IF3 activity and a modified L-phenylalanine: tRNAPhe ligase (EC 6.1.1.20) activity. These two defects cotransduce and are located near 38 min on the new E. coli map. Thermoresistant revertants showing in vitro reversion for one defect also revert in vitro for the other defect. The thermosensitive mutation is recessive to its wild type allele, and in vitro analysis of wild type/ mutant heterodiploïds also show reversion for both defects.

MeSH Terms
Amino Acyl-tRNA Synthetases/metabolism Chromosome Mapping Chromosomes, Bacterial Escherichia coli/enzymology,metabolism Genetic Complementation Test Hot Temperature Mutation Peptide Initiation Factors/metabolism Phenylalanine-tRNA Ligase/metabolism Protein Biosynthesis Transduction, Genetic
Chemicals
Peptide Initiation Factors Amino Acyl-tRNA Synthetases Phenylalanine-tRNA Ligase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Springer M
Graffe M
Grunberg-Manago M
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24 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00446908
Published
1977-02-28
Pages
17-26
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00446908
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