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PMID: 3283108 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Near-UV stress in Salmonella typhimurium: 4-thiouridine in tRNA, ppGpp, and ApppGpp as components of an adaptive response.

Journal of bacteriology ·Vol. 170 ·No. 5 ·1988-05-00 ·Pages 2344-51

Kramer GF, Baker JC, Ames BN

Abstract

We have examined the role of 4-thiouridine in the responses of Salmonella typhimurium to near-UV irradiation. Mutants lacking 4-thiouridine (nuv) and mutants defective in the synthesis of ppGpp (guanosine 5'-diphosphate-3'-diphosphate) (relA) were found to be sensitive to killing by near-UV. Near-UV induced the synthesis of a set of proteins that were not induced in the nuv mutant. Some of these proteins were identified as oxidative defense proteins, and others were identified as ppGpp-inducible proteins. Over 100-fold increases in ApppGpp (adenosine 5', 5"'-triphosphoguanosine-3"'-diphosphate, the adenylylated form of ppGpp) were observed in wild-type cells after near-UV irradiation but not in the 4-thiouridine-deficient mutant. These data support a model in which ppGpp and ApppGpp, a dinucleotide proposed to be synthesized by tRNA-aminoacyl synthetases as a response to the cross-linking of 4-thiouridine in tRNA by near-UV, induce the synthesis of proteins necessary for resistance to near-UV irradiation.

MeSH Terms
Bacterial Proteins/biosynthesis Chromatography, High Pressure Liquid Genes, Bacterial Guanine Nucleotides/biosynthesis Guanosine Tetraphosphate/analogs & derivatives,biosynthesis,genetics Mutation RNA, Transfer/metabolism Salmonella typhimurium/genetics,growth & development,metabolism,radiation effects Thiouridine/metabolism Ultraviolet Rays
Chemicals
Bacterial Proteins Guanine Nucleotides Thiouridine Guanosine Tetraphosphate adenosine 5',5'''-triphosphoguanosine-3'''-diphosphate RNA, Transfer
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kramer G F
Department of Biochemistry, University of California, Berkeley 94720.
Baker J C
Ames B N
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.5.2344-2351.1988
Published
1988-05-00
Pages
2344-51
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211128
Subset
IM
Grants
NCI NIH HHS · CA39910 · United States
NIEHS NIH HHS · ES01896 · United States
NIGMS NIH HHS · GM19993 · United States
External Links
PubMed source DOI: 10.1128/jb.170.5.2344-2351.1988 PMC Full Text View journal details
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