Biochemical bases for the antimetabolite action of L-serine hydroxamate.

Journal of bacteriology ·Vol. 106 ·No. 3 ·1971-06-00 ·Pages 972-82

Tosa T, Pizer LI

Abstract

The amino acid analogue l-serine hydroxamate, which is bacteriostatic for Escherichia coli, has been shown to inhibit protein synthesis. The antimetabolite is a competitive inhibitor of seryl-transfer ribonucleic acid (tRNA) synthetase with a K(i) value of 30 mum. Mutants resistant to l-serine hydroxamate have been selected, and three were shown to have seryl-tRNA synthetases with increased K(i) values. One mutant contains a 3-phosphoglycerate dehydrogenase which is insensitive to inhibition by l-serine.

MeSH Terms

Bacterial Proteins/biosynthesis Carbon Isotopes Culture Media Drug Resistance, Microbial Escherichia coli/drug effects,enzymology,growth & development,metabolism Genetics, Microbial Glycerolphosphate Dehydrogenase/metabolism Glycine/metabolism Hydroxamic Acids/pharmacology Leucine/metabolism Ligases/antagonists & inhibitors,metabolism Mutagens Mutation Nitrosoguanidines RNA, Bacterial/biosynthesis Serine/metabolism,pharmacology Spectrophotometry Stereoisomerism Threonine/metabolism Tritium Ultracentrifugation Uracil/metabolism

Chemicals

Bacterial Proteins Carbon Isotopes Culture Media Hydroxamic Acids Mutagens Nitrosoguanidines RNA, Bacterial Tritium Threonine Serine Uracil Glycerolphosphate Dehydrogenase Ligases Leucine Glycine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Tosa T

Pizer L I

References (18)

18 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.106.3.972-982.1971

Published

1971-06-00

Pages

972-82

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC248741

Subset

External Links

PubMed source DOI: 10.1128/jb.106.3.972-982.1971 PMC Full Text View journal details

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Biochemical bases for the antimetabolite action of L-serine hydroxamate.

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