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PMID: 783151 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Method for the isolation of Escherichia coli relaxed mutants, utilizing near-ultraviolet irradiation.

Journal of bacteriology ·Vol. 127 ·No. 3 ·1976-09-00 ·Pages 1587-9

Ramabhadran TV

Abstract

Near-ultraviolet radiation (300 to 380 nm) induces a transient growth inhibition in "stringent" (rel+) strains of Escherichia coli, whereas "relaxed" (rel-) strains are only mildly affected. This difference permits rapid isolation of large numbers of relaxed mutants of E. coli.

MeSH Terms

Bacteriological Techniques Escherichia coli/growth & development,radiation effects Mutation Ultraviolet Rays

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Ramabhadran T V

References (7)

7 references, click to expand

A genetic locus for the regulation of ribonucleic acid synthesis.
Proc Natl Acad Sci U S A. 1961 Dec 15;47:2005-14 PMID: 13916843
Induction by cyanide, dinitrophenol, and trinitrophenol of growth inhibition and of recovery from far-ultraviolet killing in Escherichia coli B: relationship to photoprotection.
Radiat Res. 1972 Mar;49(3):631-46 PMID: 4552035
Isolation of "relaxed" mutants of Escherichia coli.
J Bacteriol. 1968 Feb;95(2):729-31 PMID: 4867755
Polarity in relaxed strains of Salmonella typhimurium.
J Mol Biol. 1968 Jan 14;31(1):127-34 PMID: 4866110
Mechanism of growth delay induced in Escherichia coli by near ultraviolet radiation.
Proc Natl Acad Sci U S A. 1976 Jan;73(1):59-63 PMID: 1108019
Effects of near-ultraviolet and violet radiations (313-405 nm) on DNA, RNA, and protein synthesis in E. coli B/r: implications for growth delay.
Photochem Photobiol. 1975 Sep-Oct;22(3-4):117-23 PMID: 1105605
In vivo induction of 4-thiouridine-cytidine adducts in tRNA of E. coli B/r by near-ultraviolet radiation.
Photochem Photobiol. 1976 May;23(5):315-21 PMID: 778865

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.127.3.1587-1589.1976

Published

1976-09-00

Pages

1587-9

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC232962

Subset

External Links

PubMed source DOI: 10.1128/jb.127.3.1587-1589.1976 PMC Full Text View journal details

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Method for the isolation of Escherichia coli relaxed mutants, utilizing near-ultraviolet irradiation.

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