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PMID: 789361 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Escherichia coli mutant lacking 4-thiouridine in its transfer ribonucleic acid.

Journal of bacteriology ·Vol. 128 ·No. 2 ·1976-11-00 ·Pages 671-2

Ramabhadran TV, Fossum T, Jagger J

Abstract

A mutant of Escherichia coli has been isolated that lacks 4-thiouridine, a rare base in transfer ribonucleic acid. The mutant grows at the same rate as wild-type cells. It shows little near-ultraviolet-induced growth delay, thus supporting earlier hypotheses that 4-thiouridine in transfer ribonucleic acid is the chromophore for this growth delay.

MeSH Terms

Escherichia coli/analysis,growth & development Mutation RNA, Bacterial/analysis RNA, Transfer/analysis Thiouridine/analysis

Chemicals

RNA, Bacterial Thiouridine RNA, Transfer

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Ramabhadran T V

Fossum T

Jagger J

References (11)

11 references, click to expand

Conformation and functioning of tRNAs: cross-linked tRNAs as substrate for tRNA nucleotidyl-transferase and aminoacyl synthetases.
Biochimie. 1974;56(8):1089-101 PMID: 4614866
In vivo induction of 4-thiouridine-cytidine adducts in tRNA of E. coli B/r by near-ultraviolet radiation.
Photochem Photobiol. 1976 May;23(5):315-21 PMID: 778865
The isolation of 4-thiouridylic acid from the soluble ribonucleic acid of Escherichia coli.
J Biol Chem. 1965 Oct;240(10):3975-8 PMID: 5320644
4-Thiouridine as the target for near-ultraviolet light induced growth delay in Escherichia coli.
Biochem Biophys Res Commun. 1975 Oct 27;66(4):1454-61 PMID: 1103892
Introduction of an intramolecular flourescent pobe in E. coli tRNA(Val)(1).
FEBS Lett. 1971 Oct 1;17(2):236-240 PMID: 11946036
Mechanism of growth delay induced in Escherichia coli by near ultraviolet radiation.
Proc Natl Acad Sci U S A. 1976 Jan;73(1):59-63 PMID: 1108019
Photochemistry of 4-thiouridine in Escherichia coli transfer RNA1Val.
J Mol Biol. 1971 May 28;58(1):367-79 PMID: 4932656
Method for the isolation of Escherichia coli relaxed mutants, utilizing near-ultraviolet irradiation.
J Bacteriol. 1976 Sep;127(3):1587-9 PMID: 783151
Studies on polynucleotides. CI. Escherichia coli tyrosine and formylmethionine transfer ribonucleic acids: effect of chemical modification of 4-thiouridine to uridine on their biological properties.
J Biol Chem. 1972 Aug 10;247(15):4879-92 PMID: 4340704
Selective inactivation of amino acid acceptor and ribosome-binding activities of Escherichia coli tRNA by modification with cyanogen bromide.
Biochim Biophys Acta. 1971 Aug 12;246(1):123-31 PMID: 4941745
Effects of near-ultraviolet and violet radiations (313-405 nm) on DNA, RNA, and protein synthesis in E. coli B/r: implications for growth delay.
Photochem Photobiol. 1975 Sep-Oct;22(3-4):117-23 PMID: 1105605

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.128.2.671-672.1976

Published

1976-11-00

Pages

671-2

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC232808

Subset

External Links

PubMed source DOI: 10.1128/jb.128.2.671-672.1976 PMC Full Text View journal details

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Escherichia coli mutant lacking 4-thiouridine in its transfer ribonucleic acid.

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