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PMID: 3186699 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Recognition helices of lac and lambda repressor are oriented in opposite directions and recognize similar DNA sequences.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 21 ·1988-11-00 ·Pages 7947-51

Lehming N, Sartorius J, Oehler S, von Wilcken-Bergmann B, Müller-Hill B

Abstract

Exchanges in positions 1 and 2 of the putative recognition helix allow lac repressor to bind to ideal lac operator variants in which base pair 4 has been replaced. We show here that an Arg-22----Asn exchange in position 6 of the putative recognition helix of lac repressor abolishes lac repressor binding to ideal lac operator. This lac repressor variant, however, binds to a variant of the ideal lac operator 5' TTTGAGCGCTCAAA 3' in which the original G.C of position 6 has been replaced by T.A. This result and our previous data confirm our suggestion that the N terminus of the recognition helix of lac repressor enters the major groove close to the center of symmetry of lac operator and that its C terminus leaves the major groove further away from the center of symmetry. The consequences of this model are discussed in regard to various phage and bacterial repressor operator systems.

MeSH Terms
Base Sequence DNA, Bacterial/analysis DNA-Binding Proteins Magnetic Resonance Spectroscopy Molecular Sequence Data Plasmids Repressor Proteins/analysis Transcription Factors/analysis Viral Proteins Viral Regulatory and Accessory Proteins
Chemicals
DNA, Bacterial DNA-Binding Proteins Repressor Proteins Transcription Factors Viral Proteins Viral Regulatory and Accessory Proteins phage repressor proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lehming N
Institut für Genetik, Universität zu Köln, Federal Republic of Germany.
Sartorius J
Oehler S
von Wilcken-Bergmann B
Müller-Hill B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.21.7947
Published
1988-11-00
Pages
7947-51
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282330
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.85.21.7947 PMC Full Text View journal details
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