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PMID: 6280140 Published · ppublish English Journal Article

Molecular cloning and nucleotide sequencing of the gene for E. coli cAMP receptor protein.

Nucleic acids research ·Vol. 10 ·No. 4 ·1982-02-25 ·Pages 1345-61

Aiba H, Fujimoto S, Ozaki N

Abstract

The crp gene of E. coli, which codes for cAMP receptor protein (CRP), has been cloned in the plasmid pBR322 on the basis of a genetic complementation. One of the recombinant plasmids, pHA1, was shown to direct the synthesis of CRP in a cell-free system. The location of the crp gene was determined by constructing subclones carrying various portions of pHA1. The nucleotide sequence of the crp gene has been determined. The coding region consists of 627 base pairs (bp), which specify a protein of 209 amino acids. The predicted amino acid sequence from the DNA sequence is consistent with the amino acid sequence partially known and the amino acid composition of CRP. After the coding region, there is a G-C rich inverted repeat sequence followed by a run of Ts, which could be a terminator of the crp gene. A possible promoter sequence was found about 180 bp upstream from the initiation codon and was shown to act as a promoter in vitro and in vivo. There are two dyad symmetry regions in a 167 bp leader sequence.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Cyclic AMP/metabolism DNA Restriction Enzymes DNA, Bacterial/genetics Escherichia coli/genetics,metabolism Genes Plasmids Protein Biosynthesis Receptors, Cyclic AMP/genetics,metabolism Transcription, Genetic
Chemicals
DNA, Bacterial Receptors, Cyclic AMP Cyclic AMP DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Aiba H
Fujimoto S
Ozaki N
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42 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.4.1345
Published
1982-02-25
Pages
1345-61
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320530
Subset
IM
Databases
GENBANK
J01598
External Links
PubMed source DOI: 10.1093/nar/10.4.1345 PMC Full Text View journal details
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