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PMID: 6316325 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A perfectly symmetric lac operator binds the lac repressor very tightly.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 22 ·1983-11-00 ·Pages 6785-9

Sadler JR, Sasmor H, Betz JL

Abstract

A completely symmetric DNA segment has been constructed that binds the lactose repressor of Escherichia coli 10-fold more tightly than does the natural lactose operator sequence. This tight-binding operator is an inverted repeat of a 15-base-pair segment from the left half of the natural operator sequence, the inversion being about the point indicated by the arrow shown below: (sequence in text) where the upper sequence is the natural operator and the lower sequence is the symmetric operator. The increased affinity of repressor for this symmetric sequence supports the idea that the tetrameric repressor is designed for a two-module binding to DNA, presumably via two (or two pairs) of its identical subunits. The natural operator is apparently "flawed" by "incorrect" base pairs in the right operator half and by an "incorrect" spacing between the operator halves with respect to maximal repressor binding.

MeSH Terms
Base Sequence Cloning, Molecular Escherichia coli/genetics Genetic Vectors Kinetics Lac Operon Plasmids Protein Binding Repressor Proteins/genetics Transcription Factors/genetics
Chemicals
Repressor Proteins Transcription Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sadler J R
Sasmor H
Betz J L
References (28)
28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.22.6785
Published
1983-11-00
Pages
6785-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390070
Subset
IM
Grants
NIGMS NIH HHS · GM 24606 · United States
NIGMS NIH HHS · GM 30709 · United States
Databases
GENBANK
J01636, J01637, K01483, K01793
External Links
PubMed source DOI: 10.1073/pnas.80.22.6785 PMC Full Text View journal details
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