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PMID: 3044614 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Two mRNAs that differ by two nontemplated nucleotides encode the amino coterminal proteins P and V of the paramyxovirus SV5.

Cell ·Vol. 54 ·No. 6 ·1988-09-09 ·Pages 891-902

Thomas SM, Lamb RA, Paterson RG

Abstract

The "P" gene of the paramyxovirus SV5 encodes two known proteins, P (Mr approximately equal to 44,000) and V (Mr approximately equal to 24,000). The complete nucleotide sequence of the "P" gene has been obtained and is found to contain two open reading frames, neither of which is large enough to encode the P protein. We have shown that the P and V proteins are translated from two mRNAs that differ by the presence of two nontemplated G residues in the P mRNA. These two additional nucleotides convert the two open reading frames to one of 392 amino acids. The P and V proteins are amino coterminal and have 164 amino acids in common. The unique C terminus of V consists of a cysteine-rich region that resembles a cysteine-rich metal binding domain. An open reading frame that contains this cysteine-rich region exists in all other paramyxovirus "P" gene sequences examined, which suggests that it may have important biological significance.

MeSH Terms
Amino Acid Sequence Antibodies, Monoclonal/immunology Base Sequence Cloning, Molecular DNA/genetics Genes, Viral Hydrogen Bonding Immunologic Techniques Molecular Sequence Data Nucleic Acid Conformation Paramyxoviridae/genetics RNA Processing, Post-Transcriptional RNA, Messenger/genetics,ultrastructure RNA, Viral/genetics Transcription, Genetic Viral Proteins/genetics
Chemicals
Antibodies, Monoclonal RNA, Messenger RNA, Viral Viral Proteins DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Thomas S M
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208.
Lamb R A
Paterson R G
References (65)
65 references, click to expand
  1. Analysis and gene assignment of mRNAs of a paramyxovirus, simian virus 5.
    Virology. 1984 Oct 30;138(2):310-23 PMID: 6548594
  2. Transcriptive complex of Newcastle disease virus. I. Both L and P proteins are required to constitute an active complex.
    Virology. 1983 Jul 15;128(1):105-17 PMID: 6683907
  3. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  4. The P protein and the nonstructural 38K and 29K proteins of Newcastle disease virus are derived from the same open reading frame.
    Virology. 1988 May;164(1):256-64 PMID: 3363866
  5. MULTIPLICATION OF A MYXOVIRUS (SV5) WITH MINIMAL CYTOPATHIC EFFECTS AND WITHOUT INTERFERENCE.
    Virology. 1964 Jun;23:224-33 PMID: 14192298
  6. Ability of the hydrophobic fusion-related external domain of a paramyxovirus F protein to act as a membrane anchor.
    Cell. 1987 Feb 13;48(3):441-52 PMID: 3026647
  7. T antigen repression of SV40 early transcription from two promoters.
    Cell. 1981 Dec;27(3 Pt 2):603-13 PMID: 6101224
  8. Splicing of messenger RNA precursors.
    Annu Rev Biochem. 1986;55:1119-50 PMID: 2943217
  9. Measles virus P gene codes for two proteins.
    J Virol. 1985 Mar;53(3):908-19 PMID: 3882996
  10. The 2.2 kb E1b mRNA of human Ad12 and Ad5 codes for two tumor antigens starting at different AUG triplets.
    Cell. 1981 Nov;27(1 Pt 2):121-31 PMID: 7326748
  11. Trans splicing of mRNA precursors in vitro.
    Cell. 1985 Aug;42(1):165-71 PMID: 3848348
  12. An efficient ribosomal frame-shifting signal in the polymerase-encoding region of the coronavirus IBV.
    EMBO J. 1987 Dec 1;6(12):3779-85 PMID: 3428275
  13. Molecular cloning and nucleotide sequence of P, M and F genes of Newcastle disease virus avirulent strain D26.
    Virus Res. 1987 May;7(3):241-55 PMID: 3604456
  14. Multiple functional domains in the adenovirus E1A gene.
    Cell. 1987 Jan 30;48(2):177-8 PMID: 2948653
  15. Hemagglutinin-neuraminidase protein of the paramyxovirus simian virus 5: nucleotide sequence of the mRNA predicts an N-terminal membrane anchor.
    J Virol. 1985 Apr;54(1):1-6 PMID: 3973974
  16. Sequence analysis of the P and C protein genes of human parainfluenza virus type 3: patterns of amino acid sequence homology among paramyxovirus proteins.
    J Gen Virol. 1986 Dec;67 ( Pt 12):2705-19 PMID: 3025346
  17. Tat protein from human immunodeficiency virus forms a metal-linked dimer.
    Science. 1988 Apr 1;240(4848):70-3 PMID: 2832944
  18. Isolation and characterization of monoclonal antibodies to simian virus 5 and their use in revealing antigenic differences between human, canine and simian isolates.
    J Gen Virol. 1987 Nov;68 ( Pt 11):2769-80 PMID: 2445904
  19. Sequence of 3,687 nucleotides from the 3' end of Sendai virus genome RNA and the predicted amino acid sequences of viral NP, P and C proteins.
    Nucleic Acids Res. 1983 Nov 11;11(21):7317-30 PMID: 6316257
  20. Coding assignments of the five smaller mRNAs of Newcastle disease virus.
    J Virol. 1982 Sep;43(3):1024-31 PMID: 7143562
  21. Synthesis of mumps virus polypeptides in infected Vero cells.
    Virology. 1982 Jun;119(2):430-8 PMID: 7080449
  22. Influenza B virus genome: sequences and structural organization of RNA segment 8 and the mRNAs coding for the NS1 and NS2 proteins.
    J Virol. 1982 Apr;42(1):186-93 PMID: 6283137
  23. Retroviruses.
    Science. 1988 Jun 10;240(4858):1427-35 PMID: 3287617
  24. Expression of the Rous sarcoma virus pol gene by ribosomal frameshifting.
    Science. 1985 Dec 13;230(4731):1237-42 PMID: 2416054
  25. The steroid and thyroid hormone receptor superfamily.
    Science. 1988 May 13;240(4854):889-95 PMID: 3283939
  26. A previously unrecognized influenza B virus glycoprotein from a bicistronic mRNA that also encodes the viral neuraminidase.
    Proc Natl Acad Sci U S A. 1983 Aug;80(16):4879-83 PMID: 6308656
  27. Murine leukemia virus protease is encoded by the gag-pol gene and is synthesized through suppression of an amber termination codon.
    Proc Natl Acad Sci U S A. 1985 Mar;82(6):1618-22 PMID: 3885215
  28. Sendai virus contains overlapping genes expressed from a single mRNA.
    Cell. 1983 Dec;35(3 Pt 2):829-36 PMID: 6317203
  29. A trans-spliced leader sequence on actin mRNA in C. elegans.
    Cell. 1987 Jun 19;49(6):753-61 PMID: 3581169
  30. Ribosomal initiation at alternate AUGs on the Sendai virus P/C mRNA.
    J Virol. 1986 Feb;57(2):684-7 PMID: 3003399
  31. Evidence for trans splicing in trypanosomes.
    Cell. 1986 Nov 21;47(4):527-35 PMID: 3022935
  32. Spliced and unspliced messenger RNAs synthesized from cloned influenza virus M DNA in an SV40 vector: expression of the influenza virus membrane protein (M1).
    Virology. 1982 Dec;123(2):237-56 PMID: 6184879
  33. Translational modulation in vitro of a eukaryotic viral mRNA encoding overlapping genes: ribosome scanning and potential roles of conformational changes in the P/C mRNA of Sendai virus.
    Biochem Biophys Res Commun. 1985 Aug 30;131(1):91-7 PMID: 2994668
  34. Vaccinia virus produces late mRNAs by discontinuous synthesis.
    Cell. 1987 Jul 17;50(2):153-62 PMID: 3036368
  35. Extensive editing of the cytochrome c oxidase III transcript in Trypanosoma brucei.
    Cell. 1988 May 6;53(3):413-22 PMID: 2452697
  36. Binding of mammalian ribosomes to MS2 phage RNA reveals an overlapping gene encoding a lysis function.
    Cell. 1979 Oct;18(2):247-56 PMID: 498271
  37. Evidence for in vivo trans splicing of pre-mRNAs in tobacco chloroplasts.
    Cell. 1987 Jan 16;48(1):111-9 PMID: 3791410
  38. Molecular cloning and sequence analysis of the human parainfluenza 3 virus mRNA encoding the P and C proteins.
    Virology. 1986 Nov;155(1):46-60 PMID: 3022477
  39. Major transcript of the frameshifted coxII gene from trypanosome mitochondria contains four nucleotides that are not encoded in the DNA.
    Cell. 1986 Sep 12;46(6):819-26 PMID: 3019552
  40. Apolipoprotein B-48 is the product of a messenger RNA with an organ-specific in-frame stop codon.
    Science. 1987 Oct 16;238(4825):363-6 PMID: 3659919
  41. Sequences of mRNAs derived from genome RNA segment 7 of influenza virus: colinear and interrupted mRNAs code for overlapping proteins.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4170-4 PMID: 6945577
  42. Sequence coding for the alphavirus nonstructural proteins is interrupted by an opal termination codon.
    Proc Natl Acad Sci U S A. 1983 Sep;80(17):5271-5 PMID: 6577423
  43. Editing of kinetoplastid mitochondrial mRNAs by uridine addition and deletion generates conserved amino acid sequences and AUG initiation codons.
    Cell. 1988 May 6;53(3):401-11 PMID: 2452696
  44. Nucleotide sequence of the entire protein coding region of canine distemper virus polymerase-associated (P) protein mRNA.
    Virus Res. 1985 Nov;3(4):367-72 PMID: 3000106
  45. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  46. A novel form of tissue-specific RNA processing produces apolipoprotein-B48 in intestine.
    Cell. 1987 Sep 11;50(6):831-40 PMID: 3621347
  47. Alternative splicing: a ubiquitous mechanism for the generation of multiple protein isoforms from single genes.
    Annu Rev Biochem. 1987;56:467-95 PMID: 3304142
  48. Evidence for a ninth influenza viral polypeptide.
    Virology. 1978 Nov;91(1):60-78 PMID: 726267
  49. Integration of a small integral membrane protein, M2, of influenza virus into the endoplasmic reticulum: analysis of the internal signal-anchor domain of a protein with an ectoplasmic NH2 terminus.
    J Cell Biol. 1988 May;106(5):1489-98 PMID: 2836432
  50. Mutations that inactivate a yeast transcriptional regulatory protein cluster in an evolutionarily conserved DNA binding domain.
    Proc Natl Acad Sci U S A. 1987 Apr;84(8):2401-5 PMID: 3550810
  51. Developmentally regulated addition of nucleotides within apocytochrome b transcripts in Trypanosoma brucei.
    Cell. 1987 May 8;49(3):337-45 PMID: 3568129
  52. Two efficient ribosomal frameshifting events are required for synthesis of mouse mammary tumor virus gag-related polyproteins.
    Proc Natl Acad Sci U S A. 1987 Jun;84(12):4298-302 PMID: 3035577
  53. Early events in the biosynthesis of the lysosomal enzyme cathepsin D.
    J Biol Chem. 1979 Dec 10;254(23):11771-4 PMID: 500673
  54. Discontinuous transcription or RNA processing of vaccinia virus late messengers results in a 5' poly(A) leader.
    Cell. 1987 Jul 17;50(2):163-9 PMID: 3594569
  55. Completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus.
    J Gen Virol. 1987 Jan;68 ( Pt 1):57-77 PMID: 3027249
  56. Vaccinia virus late transcripts generated in vitro have a poly(A) head.
    EMBO J. 1988 Apr;7(4):1183-90 PMID: 3402436
  57. Molecular cloning and sequence analysis of the mumps virus gene encoding the P protein: mumps virus P gene is monocistronic.
    J Gen Virol. 1988 Aug;69 ( Pt 8):2043-9 PMID: 3404121
  58. Polypeptide synthesis in simian virus 5-infected cells.
    J Virol. 1977 Jul;23(1):177-87 PMID: 196101
  59. Nucleotide sequence coding for the "signal peptide" and N terminus of the hemagglutinin from an asian (H2N2) strain of influenza virus.
    Virology. 1979 Sep;97(2):468-72 PMID: 473601
  60. Trans splicing of mRNA precursors.
    Cell. 1985 Aug;42(1):157-64 PMID: 3848347
  61. Identification of a novel Y branch structure as an intermediate in trypanosome mRNA processing: evidence for trans splicing.
    Cell. 1986 Nov 21;47(4):517-25 PMID: 3779835
  62. Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA.
    Nucleic Acids Res. 1982 Oct 25;10(20):6487-500 PMID: 6757864
  63. Sequence of interrupted and uninterrupted mRNAs and cloned DNA coding for the two overlapping nonstructural proteins of influenza virus.
    Cell. 1980 Sep;21(2):475-85 PMID: 7407920
  64. The transcriptase complex of the paramyxovirus SV5.
    Virology. 1977 Oct 15;82(2):493-508 PMID: 919349
  65. Messenger RNA encoding the phosphoprotein (P) gene of human parainfluenza virus 3 is bicistronic.
    Virology. 1986 Sep;153(2):318-25 PMID: 3016995
Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
DOI
10.1016/s0092-8674(88)91285-8
Published
1988-09-09
Pages
891-902
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC7133244
Subset
IM
Grants
NIAID NIH HHS · AI-20201 · United States
NIAID NIH HHS · AI-23173 · United States
Databases
GENBANK
J03142
External Links
PubMed source DOI: 10.1016/s0092-8674(88)91285-8 PMC Full Text View journal details
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