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PMID: 6316257 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequence of 3,687 nucleotides from the 3' end of Sendai virus genome RNA and the predicted amino acid sequences of viral NP, P and C proteins.

Nucleic acids research ·Vol. 11 ·No. 21 ·1983-11-11 ·Pages 7317-30

Shioda T, Hidaka Y, Kanda T, Shibuta H, Nomoto A, Iwasaki K

Abstract

The sequence of 3,687 nucleotides from the 3' end of the Sendai virus genome (Z strain) was determined by a molecular cloning technique followed by rapid sequence analysis. Two large open reading frames, one consisting of 1,572 nucleotides and the other of 1,704 nucleotides, were observed in the region, that is OP-1 and OP-2 from the 3' end of the genome. The amino acid sequences of the gene products were predicted from the observed sequence. Determination of amino acid compositions of viral proteins, P, HN, Fo, NP and M, led us to conclude that NP and P are the gene products of OP-1 and OP-2, respectively. An additional open reading frame consisting of 612 nucleotides (OP-3) was discovered in the 3' most proximal region of OP-2. The predicted product of OP-3 was considered to be viral non-structural protein C. The leader sequence of 51 nucleotides at the 3' terminal of the genome and consensus sequences at 3' and 5' ends of each gene for proteins NP and P were identified.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA/metabolism DNA Restriction Enzymes Genes, Viral Parainfluenza Virus 1, Human/genetics Plasmids RNA, Viral/genetics Viral Proteins/genetics
Chemicals
RNA, Viral Viral Proteins DNA DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Shioda T
Hidaka Y
Kanda T
Shibuta H
Nomoto A
Iwasaki K
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36 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.21.7317
Published
1983-11-11
Pages
7317-30
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326485
Subset
IM
Databases
GENBANK
K01146, K01703, X00087
External Links
PubMed source DOI: 10.1093/nar/11.21.7317 PMC Full Text View journal details
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