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PMID: 7050911 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The complete sequence and coding content of snowshoe hare bunyavirus small (S) viral RNA species.

Nucleic acids research ·Vol. 10 ·No. 12 ·1982-06-25 ·Pages 3703-13

Bishop DH, Gould KG, Akashi H, Clerx-van Haaster CM

Abstract

The complete sequence of the small (S) viral RNA species of snowshoe hare (SSH) bunyavirus has been determined, principally from a DNA copy of the RNA cloned in the E.coli plasmid pBr322. The viral S RNA (negative sense strand) is 982 nucleotides long (3.3 x 10(5) daltons) with complementary 5' and 3' end sequences. It has a base composition of 30.5%U, 25.8%A, 24.9%C and 18.7%G. In the viral complementary (plus sense) strand there are two overlapping open reading frames initiated by methionine codons. One reading frame codes for a 26.8 x 10(3) dalton protein, the other for a 10.5 x 10(3) dalton protein. The larger gene product is presumably related to the viral nucleoprotein (N) that is coded by the S RNA (Gentsch and Bishop (1978) J. Virol. 28, 417-419). The smaller gene product is probably related to the recently identified S RNA coded nonstructural protein (NSS) induced in virus infected cells (Fuller and Bishop (1982) J. Virol. 41, 643-648).

MeSH Terms
Amino Acid Sequence Animals Base Sequence Bunyaviridae/genetics Cloning, Molecular DNA, Recombinant/metabolism Escherichia coli/genetics Nucleic Acid Conformation Plasmids RNA, Viral/genetics Rabbits Transcription, Genetic
Chemicals
DNA, Recombinant RNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bishop D H
Gould K G
Akashi H
Clerx-van Haaster C M
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22 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.12.3703
Published
1982-06-25
Pages
3703-13
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320745
Subset
IM
Databases
GENBANK
J02390
External Links
PubMed source DOI: 10.1093/nar/10.12.3703 PMC Full Text View journal details
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