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PMID: 7041090 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The complete sequence of RNA segment 2 of influenza A/NT/60/68 P1 protein.

Nucleic acids research ·Vol. 10 ·No. 4 ·1982-02-25 ·Pages 1335-43

Bishop DH, Huddleston JA, Brownlee GG

Abstract

A DNA copy of influenza A/NT/60/68 viral RNA segment 2, corresponding to protein P1, has been cloned in the E.coli plasmid pBr322. The clone is 2341 nucleotides long and represents a full-length copy of the viral RNA. In the viral complementary (plus sense) strand there is an open reading frame that is 2271 nucleotides long. The predicted primary gene product is a basic 86,300 dalton protein with a net charge at neutral pH of +23. A 29 amino acid stretch of the protein (coded by nucleotide residues 583-669) is highly basic and contains 7 lysine and 8 arginine residues. Other smaller clusters of basic amino acids are also present in the protein.

MeSH Terms
Amino Acid Sequence Base Composition Base Sequence Cloning, Molecular DNA Escherichia coli/genetics Influenza A virus/genetics Molecular Weight Plasmids RNA, Viral/genetics Viral Proteins/genetics
Chemicals
RNA, Viral Viral Proteins DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bishop D H
Huddleston J A
Brownlee G G
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27 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.4.1335
Published
1982-02-25
Pages
1335-43
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320529
Subset
IM
Grants
PHS HHS · A1 14183 · United States
Databases
GENBANK
J02138
External Links
PubMed source DOI: 10.1093/nar/10.4.1335 PMC Full Text View journal details
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