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PMID: 7465426 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Complete nucleotide sequence of the influenza A/PR/8/34 virus NS gene and comparison with the NS genes of the A/Udorn/72 and A/FPV/Rostock/34 strains.

Nucleic acids research ·Vol. 8 ·No. 23 ·1980-12-11 ·Pages 5845-58

Baez M, Taussig R, Zazra JJ, Young JF, Palese P, Reisfeld A, Skalka AM

Abstract

The nucleotide sequence of the NS gene of the human influenza virus A/PR/8/34 was determined and found to be the same length (890 nucleotides) as the NS gene of another human influenza virus A/Udorn/72 and of the avian isolate A/FPV/Rostock/34. Comparison of the sequences of the NS genes of the two human influenza viruses shows an 8.9% difference whereas the NS gene of the avian isolate differs by only 8% from that of the human strain A/PR/8/34. The extensive sequence similarity among these three genes does not support the notion of species specific homology groups among NS genes of avian and human influenza virus strains. The primary sequence of the A/PR/8/34 NS gene is consistent with the findings that the influenza virus NS gene may code for two overlapping polypeptides. In addition, an open reading frame potentially coding for a polypeptide 167 amino acids in length was found in the negative strand RNA of the A/PR/8/34 virus NS gene.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Genes, Viral Influenza A virus/genetics Peptides/genetics RNA, Viral/genetics Species Specificity
Chemicals
Peptides RNA, Viral
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Baez M
Taussig R
Zazra J J
Young J F
Palese P
Reisfeld A
Skalka A M
References (42)
42 references, click to expand
  1. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
    Gene. 1979 May;6(1):23-8 PMID: 383576
  2. 5' and 3' terminal nucleotide sequences of the RNA genome segments of influenza virus.
    Nucleic Acids Res. 1979 Aug 24;6(12):3745-57 PMID: 493121
  3. The smallest genome RNA segment of influenza virus contains two genes that may overlap.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3790-4 PMID: 291039
  4. Complete nucleotide sequence of an influenza virus haemagglutinin gene from cloned DNA.
    Nature. 1979 Nov 29;282(5738):471-7 PMID: 503226
  5. Segment 8 of the influenza virus genome is unique in coding for two polypeptides.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):4908-12 PMID: 291907
  6. Evolution of human influenza A viruses in nature: sequential mutations in the genomes of new H1N1.
    Cell. 1979 Sep;18(1):73-83 PMID: 509526
  7. The genome of human papovavirus BKV.
    Cell. 1979 Dec;18(4):963-77 PMID: 229976
  8. Evolution of human influenza A viruses in nature: recombination contributes to genetic variation of H1N1 strains.
    Proc Natl Acad Sci U S A. 1979 Dec;76(12):6547-51 PMID: 293742
  9. The 3' and 5'-terminal sequences of influenza A, B and C virus RNA segments are highly conserved and show partial inverted complementarity.
    Gene. 1980 Feb;8(3):315-28 PMID: 7358274
  10. Complete structure of the hemagglutinin gene from the human influenza A/Victoria/3/75 (H3N2) strain as determined from cloned DNA.
    Cell. 1980 Mar;19(3):683-96 PMID: 6153930
  11. Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper.
    Methods Enzymol. 1979;68:220-42 PMID: 94421
  12. Plasmids of Escherichia coli as cloning vectors.
    Methods Enzymol. 1979;68:245-67 PMID: 232214
  13. Genetic relatedness of the gene which codes for the nonstructural (NS) protein of different influenza A strains.
    Virology. 1980 Apr 15;102(1):13-20 PMID: 7368565
  14. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  15. Mapping of the two overlapping genes for polypeptides NS1 and NS2 on RNA segment 8 of influenza virus genome.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):1857-61 PMID: 6246509
  16. Gene 8 of influenza virus: sequences of cDNA transcribed from the 3' ends of viral RNA of influenza A and B strains.
    Virology. 1980 Jun;103(2):291-8 PMID: 7385583
  17. Antigenic drift between the haemagglutinin of the Hong Kong influenza strains A/Aichi/2/68 and A/Victoria/3/75.
    Nature. 1980 Aug 21;286(5775):771-6 PMID: 7402351
  18. Sequence of interrupted and uninterrupted mRNAs and cloned DNA coding for the two overlapping nonstructural proteins of influenza virus.
    Cell. 1980 Sep;21(2):475-85 PMID: 7407920
  19. A rapid enzymatic DNA sequencing technique: determination of sequence alterations in early simian virus 40 temperature sensitive and deletion mutants.
    Nucleic Acids Res. 1980 May 24;8(10):2225-40 PMID: 6253881
  20. A strategy of DNA sequencing employing computer programs.
    Nucleic Acids Res. 1979 Jun 11;6(7):2601-10 PMID: 461197
  21. Nucleotide sequence of the rightward operator of phage lambda.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 PMID: 1055375
  22. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  23. Differences in RNA patterns of influenza A viruses.
    J Virol. 1976 Mar;17(3):876-84 PMID: 1255862
  24. Transcriptase activity and genome composition of defective influenza virus.
    J Virol. 1976 Apr;18(1):365-9 PMID: 1255876
  25. A reexamination of influenza single-and double-stranded RNAs by gel electrophoresis.
    Virology. 1976 Feb;69(2):789-92 PMID: 1258370
  26. Mapping of the influenza virus genome: identification of the hemagglutinin and the neuraminidase genes.
    Proc Natl Acad Sci U S A. 1976 Jun;73(6):2142-6 PMID: 1064882
  27. Influenza virus genome consists of eight distinct RNA species.
    Proc Natl Acad Sci U S A. 1976 Sep;73(9):3045-9 PMID: 1067600
  28. Mapping of the influenza virus genome. III. Identification of genes coding for nucleoprotein, membrane protein, and nonstructural protein.
    J Virol. 1976 Oct;20(1):307-13 PMID: 985644
  29. Correlation between RNA fragments of fowl plague virus and their corresponding gene functions.
    Virology. 1976 Oct 15;74(2):332-44 PMID: 982830
  30. Polypeptides specified by the influenza virus genome I. Evidence for eight distinct gene products specified by fowl plague virus.
    Virology. 1976 Oct 15;74(2):489-503 PMID: 982839
  31. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  32. Polypeptides specified by the influenza virus genoma. 2. Assignement of protein coding functions to individual genome segments by in vitro translation.
    Virology. 1977 May 15;78(2):522-36 PMID: 867816
  33. In vitro packaging of a lambda Dam vector containing EcoRI DNA fragments of Escherichia coli and phage P1.
    Gene. 1977 May;1(3-4):255-80 PMID: 338419
  34. Sequence data handling by computer.
    Nucleic Acids Res. 1977 Nov;4(11):4037-51 PMID: 593900
  35. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  36. Further procedures for sequence analysis by computer.
    Nucleic Acids Res. 1978 Mar;5(3):1013-6 PMID: 643613
  37. Nucleotide sequences at the 5' termini of influenza virus RNAs and their transcripts.
    Nucleic Acids Res. 1978 Apr;5(4):1207-19 PMID: 652519
  38. On the origin of the human influenza virus subtypes H2N2 and H3N2.
    Virology. 1978 Jun 1;87(1):13-20 PMID: 664248
  39. Biochemical evidence that "new" influenza virus strains in nature may arise by recombination (reassortment).
    Proc Natl Acad Sci U S A. 1978 Jul;75(7):3341-5 PMID: 277933
  40. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  41. Evidence for a ninth influenza viral polypeptide.
    Virology. 1978 Nov;91(1):60-78 PMID: 726267
  42. Polyadenylation and reverse transcription of influenza viral RNA.
    Nucleic Acids Res. 1979 Apr;6(4):1221-39 PMID: 88038
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.23.5845
Published
1980-12-11
Pages
5845-58
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324346
Subset
IM
Grants
NIAID NIH HHS · AI-11823 · United States
Databases
GENBANK
J02150
External Links
PubMed source DOI: 10.1093/nar/8.23.5845 PMC Full Text View journal details
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