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PMID: 461197 Published · ppublish English Journal Article

A strategy of DNA sequencing employing computer programs.

Nucleic acids research ·Vol. 6 ·No. 7 ·1979-06-11 ·Pages 2601-10

Staden R

Abstract

With modern fast sequencing techniques and suitable computer programs it is now possible to sequence whole genomes without the need of restriction maps. This paper describes computer programs that can be used to order both sequence gel readings and clones. A method of coding for uncertainties in gel readings is described. These programs are available on request.

MeSH Terms
Base Sequence Computers DNA DNA, Recombinant Oligodeoxyribonucleotides/analysis
Chemicals
DNA, Recombinant Oligodeoxyribonucleotides DNA
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Staden R
References (8)
8 references, click to expand
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  2. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  3. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  4. Further procedures for sequence analysis by computer.
    Nucleic Acids Res. 1978 Mar;5(3):1013-6 PMID: 643613
  5. The use of thin acrylamide gels for DNA sequencing.
    FEBS Lett. 1978 Mar 1;87(1):107-10 PMID: 631324
  6. Sequence data handling by computer.
    Nucleic Acids Res. 1977 Nov;4(11):4037-51 PMID: 593900
  7. Nucleotide sequence of bacteriophage phi X174 DNA.
    Nature. 1977 Feb 24;265(5596):687-95 PMID: 870828
  8. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase.
    J Mol Biol. 1975 May 25;94(3):441-8 PMID: 1100841
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
Published
1979-06-11
Pages
2601-10
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327874
Subset
IM
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