Polyadenylation and reverse transcription of influenza viral RNA.

Nucleic acids research ·Vol. 6 ·No. 4 ·1979-04-00 ·Pages 1221-39

Emtage JS, Catlin GH, Carey NH

Abstract

The polyadenylation of Fowl Plague Viral RNA and of Influenza A/Victoria Viral RNA using E. coli poly (A) polymerase and the subsequent reverse transcription of the polyadenylated species is reported. We have shown that all 8 genome fragments are adenylated and that an average of 25--30 adenylic acid residues per molecule is sufficient for maximal transcription with reverse transcriptase. The cDNA product is 95% sensitive to Sl-nuclease and hybridisation analysis against viral RNA reveals it to be a faithful copy of the RNA. Amongst the transcription products are long, discrete copies of genes 1--8, the lengths of which are comparable with those of the vRNA determined by electrophoresis on formamide acrylamide gels. These single-stranded cDNAs have been further transcribed to form double-stranded products with hair-pin structures at one end. Analysis of this material on native acrylamide gels revealed some DNA bands corresponding to the predicted sizes for genes 4--8.

MeSH Terms

DNA, Viral/biosynthesis Escherichia coli/enzymology Influenza A virus Kinetics Molecular Weight Nucleic Acid Hybridization Nucleotidyltransferases/metabolism Orthomyxoviridae Poly A/biosynthesis Polynucleotide Adenylyltransferase/metabolism RNA, Viral/metabolism RNA-Directed DNA Polymerase/metabolism

Chemicals

DNA, Viral RNA, Viral Poly A Nucleotidyltransferases Polynucleotide Adenylyltransferase RNA-Directed DNA Polymerase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Emtage J S

Catlin G H

Carey N H

References (24)

24 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/6.4.1221

Published

1979-04-00

Pages

1221-39

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327769

Subset

External Links

PubMed source DOI: 10.1093/nar/6.4.1221 PMC Full Text View journal details

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Polyadenylation and reverse transcription of influenza viral RNA.

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