Home LiteratureArticle Details
PMID: 291039 Published · ppublish English Journal Article

The smallest genome RNA segment of influenza virus contains two genes that may overlap.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 8 ·1979-08-00 ·Pages 3790-4

Inglis SC, Barrett T, Brown CM, Almond JW

Abstract

The genome of influenza virus consists of eight segments of single-stranded RNA, each of which encodes a different polypeptide. In addition to the eight recognized gene products, the virus specifies a distinct smaller nonstructural polypeptide (NS2), which is translated from a separate species of virus-specific mRNA. The location on the virus genome of the gene encoding this polypeptide was investigated by hybridization of the NS2 mRNA with isolated subgenomic RNA species, and by correlation of the inheritance of a strain-specific NS2 with inheritance of particular genome RNA segments during recombination between two different virus strains. The genetic information for NS2 was found to reside in the smallest genome RNA segment of the virion, which also encodes the NS1 polypeptide. Considering the sizes of the molecules involved, it is likely that the coding sequences for the two polypeptides overlap.

MeSH Terms
Genes, Viral Genetic Linkage Influenza A virus/genetics Molecular Weight Nucleic Acid Hybridization RNA, Messenger/genetics RNA, Viral/genetics Recombination, Genetic Viral Proteins/genetics
Chemicals
RNA, Messenger RNA, Viral Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Inglis S C
Barrett T
Brown C M
Almond J W
References (31)
31 references, click to expand
  1. Transfer of 5'-terminal cap of globin mRNA to influenza viral complementary RNA during transcription in vitro.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1618-22 PMID: 287003
  2. A single gene determines the host range of influenza virus.
    Nature. 1977 Dec 15;270(5638):617-8 PMID: 593388
  3. Polypeptides specified by the influenza virus genome. 3. Control of synthesis in infected cells.
    Virology. 1979 May;95(1):154-64 PMID: 442539
  4. Temperature-sensitive mutants of fowl plague virus: isolation and genetic characterization.
    Virology. 1979 Jan 30;92(2):416-27 PMID: 425322
  5. Genetic recombination between two strains of fowl plague virus: construction of genetic maps.
    Virology. 1979 Jan 30;92(2):407-15 PMID: 425321
  6. Nucleotide sequences at the 5' termini of influenza virus RNAs and their transcripts.
    Nucleic Acids Res. 1978 Apr;5(4):1207-19 PMID: 652519
  7. Evidence for a ninth influenza viral polypeptide.
    Virology. 1978 Nov;91(1):60-78 PMID: 726267
  8. Synthesis of influenza virus proteins in infected cells: translation of viral polypeptides, including three P polypeptides, from RNA produced by primary transcription.
    Virology. 1976 Oct 15;74(2):504-19 PMID: 982840
  9. Polypeptides specified by the influenza virus genome I. Evidence for eight distinct gene products specified by fowl plague virus.
    Virology. 1976 Oct 15;74(2):489-503 PMID: 982839
  10. Correlation between RNA fragments of fowl plague virus and their corresponding gene functions.
    Virology. 1976 Oct 15;74(2):332-44 PMID: 982830
  11. Influenza virus genome consists of eight distinct RNA species.
    Proc Natl Acad Sci U S A. 1976 Sep;73(9):3045-9 PMID: 1067600
  12. Characterization of virus-specific messenger RNAs from avian fibroblasts infected with fowl plague virus.
    J Gen Virol. 1977 Aug;36(2):237-48 PMID: 561162
  13. Mapping of the influenza virus genome. III. Identification of genes coding for nucleoprotein, membrane protein, and nonstructural protein.
    J Virol. 1976 Oct;20(1):307-13 PMID: 985644
  14. Polypeptides specified by the influenza virus genoma. 2. Assignement of protein coding functions to individual genome segments by in vitro translation.
    Virology. 1977 May 15;78(2):522-36 PMID: 867816
  15. Electron microscopic evidence for splicing of SV40 late mRNAs.
    Cell. 1978 Apr;13(4):783-90 PMID: 207441
  16. The size of Rous sarcoma virus mRNAs active in cell-free translation.
    Nature. 1977 Aug 4;268(5619):416-20 PMID: 197417
  17. Overlapping of the VP2-VP3 gene and the VP1 gene in the SV40 genome.
    Cell. 1977 Oct;12(2):529-38 PMID: 199354
  18. Location of the sequences coding for capsid proteins VP1 and VP2 on polyoma virus DNA.
    Cell. 1976 Nov;9(3):481-7 PMID: 186193
  19. Gene K, a new overlapping gene in bacteriophage G4.
    Nature. 1978 Apr 6;272(5653):510-5 PMID: 692656
  20. An amazing sequence arrangement at the 5' ends of adenovirus 2 messenger RNA.
    Cell. 1977 Sep;12(1):1-8 PMID: 902310
  21. Overlapping genes in bacteriophage phiX174.
    Nature. 1976 Nov 4;264(5581):34-41 PMID: 1004533
  22. Polypeptide synthesis in influenza virus-infected cells.
    Virology. 1972 Jul;49(1):23-36 PMID: 4625019
  23. Deoxyribonucleic acid-dependent ribonucleic acid polymerase activity in cells infected with influenza virus.
    J Virol. 1968 Jan;2(1):33-9 PMID: 5742027
  24. Inhibition of synthesis of influenza virus proteins: evidence of two host-cell-dependent events during multiplication.
    Virology. 1975 Sep;67(1):114-23 PMID: 51536
  25. Characterization of the mRNA of influenza virus.
    J Virol. 1975 Dec;16(6):1435-43 PMID: 1202243
  26. Translation of brome mosaic viral ribonucleic acid in a cell-free system derived from wheat embryo.
    Proc Natl Acad Sci U S A. 1973 Jun;70(6):1799-803 PMID: 4515941
  27. Messenger RNA for the coat protein of tobacco mosaic virus.
    Nature. 1976 Apr 29;260(5554):759-64 PMID: 1264250
  28. Differences in RNA patterns of influenza A viruses.
    J Virol. 1976 Mar;17(3):876-84 PMID: 1255862
  29. A reexamination of influenza single-and double-stranded RNAs by gel electrophoresis.
    Virology. 1976 Feb;69(2):789-92 PMID: 1258370
  30. Purification of influenza viral complementary RNA: its genetic content and activity in wheat germ cell-free extracts.
    J Virol. 1975 Dec;16(6):1464-75 PMID: 1202245
  31. Initiation sites for translation of sindbis virus 42S and 26S messenger RNAs.
    Cell. 1975 Oct;6(2):215-22 PMID: 1182802
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.8.3790
Published
1979-08-00
Pages
3790-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383920
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.8.3790 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2