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PMID: 6254054 Published · ppublish English Journal Article

Nucleotide sequence of influenza virus RNA segment 8 indicates that coding regions for NS1 and NS2 proteins overlap.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 9 ·1980-09-00 ·Pages 5074-8

Porter AG, Smith JC, Emtage JS

Abstract

The smallest RNA segment of influenza A viruses (vRNA segment 8) has recently been shown to code for two unrelated nonstructural proteins (NS1 and NS2) translated from separate mRNAs. Molecular weight considerations indicated that there might not be enough space on vRNA segment 8 for the two coding regions unless they overlap. We have recently cloned in bacterial plasmids several genes of an avian influenza A virus, fowl plague virus (EPV), and now present the complete nucleotide sequence of FPV RNA segment 8 largely determined from the cloned DNA. The DNA sequence predicts two open protein synthesis reading frames that can be translated into polypeptides of sizes similar to those of NS1 and NS2. The coding regions for these polypeptides overlap by the equivalent of 43-60 amino acids, the exact amount depending on which of several possible methionines initiates the synthesis of NS2.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Codon/genetics DNA Restriction Enzymes Genes Genes, Synthetic Genes, Viral Influenza A virus/genetics Molecular Sequence Data Molecular Weight Plasmids RNA, Viral/genetics Viral Nonstructural Proteins/genetics Viral Proteins/genetics
Chemicals
Codon NS2 protein, influenza virus A RNA, Viral Viral Nonstructural Proteins Viral Proteins DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Porter A G
Smith J C
Emtage J S
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37 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.9.5074
Published
1980-09-00
Pages
5074-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349998
Subset
IM
Databases
GENBANK
J02118, M16562
External Links
PubMed source DOI: 10.1073/pnas.77.9.5074 PMC Full Text View journal details
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