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PMID: 3034856 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Rhizobium meliloti ntrA (rpoN) gene is required for diverse metabolic functions.

Journal of bacteriology ·Vol. 169 ·No. 6 ·1987-06-00 ·Pages 2424-31

Ronson CW, Nixon BT, Albright LM, Ausubel FM

Abstract

We report the identification and cloning of an ntrA-like (glnF rpoN) gene of Rhizobium meliloti and show that the R. meliloti ntrA product (NtrA) is required for C4-dicarboxylate transport as well as for nitrate assimilation and symbiotic nitrogen fixation. DNA sequence analysis showed that R. meliloti NtrA is 38% homologous with Klebsiella pneumoniae NtrA. Subcloning and complementation analysis suggested that the R. meliloti ntrA promoter lies within 125 base pairs of the initiation codon and may be constitutively expressed.

MeSH Terms
Amino Acid Sequence Base Sequence Biological Transport Chromosome Mapping Cloning, Molecular DNA Transposable Elements DNA-Directed RNA Polymerases/physiology Dicarboxylic Acids/metabolism Gene Expression Regulation Genes, Bacterial Genetic Complementation Test Mutation Nitrogen/metabolism Phenotype Promoter Regions, Genetic Rhizobium/genetics Sigma Factor/genetics Transcription Factors/genetics
Chemicals
DNA Transposable Elements Dicarboxylic Acids Sigma Factor Transcription Factors DNA-Directed RNA Polymerases Nitrogen
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ronson C W
Nixon B T
Albright L M
Ausubel F M
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39 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.6.2424-2431.1987
Published
1987-06-00
Pages
2424-31
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212082
Subset
IM
Databases
GENBANK
M16513
External Links
PubMed source DOI: 10.1128/jb.169.6.2424-2431.1987 PMC Full Text View journal details
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