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PMID: 3008100 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA sequence of Rhizobium trifolii nodulation genes reveals a reiterated and potentially regulatory sequence preceding nodABC and nodFE.

Nucleic acids research ·Vol. 14 ·No. 7 ·1986-04-11 ·Pages 2891-903

Schofield PR, Watson JM

Abstract

The Rhizobium trifolii nod genes required for host-specific nodulation of clovers are located on 14 kb of Sym (symbiotic) plasmid DNA. Analysis of the nucleotide sequence of a 3.7 kb portion of this region has revealed open reading frames corresponding to the nodABCDEF genes. A DNA sequencing technique, using primer extension from within Tn5, has been used to determine the precise locations of Tn5 mutations within the nod genes and the phenotypes of the corresponding mutants correlate with their mapped locations. The predicted nodA and nodB genes overlap by four nucleotides and the nod F and nodE genes overlap by a single nucleotide, suggesting that translational coupling may ensure the synthesis of equimolar amounts of these gene products. The nodABC and nodFE genes constitute separate transcriptional units and each is preceded by a conserved 76-bp sequence which may be involved in the regulation of expression of these genes.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/analysis Base Sequence DNA, Bacterial/analysis Gene Expression Regulation Mutation Plasmids Repetitive Sequences, Nucleic Acid Rhizobium/genetics
Chemicals
Bacterial Proteins DNA, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schofield P R
Watson J M
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22 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.7.2891
Published
1986-04-11
Pages
2891-903
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339710
Subset
IM
Databases
GENBANK
X03721
External Links
PubMed source DOI: 10.1093/nar/14.7.2891 PMC Full Text View journal details
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