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PMID: 6094513 Published · ppublish English Journal Article

Molecular cloning and genetic organization of C4-dicarboxylate transport genes from Rhizobium leguminosarum.

Journal of bacteriology ·Vol. 160 ·No. 3 ·1984-12-00 ·Pages 903-9

Ronson CW, Astwood PM, Downie JA

Abstract

Cosmids containing C4-dicarboxylate transport (dct) genes were identified from a gene bank of Rhizobium leguminosarum DNA made in the broad-host-range vector pLAFR1 by their ability to complement R. trifolii dct mutants. The dct genes were further characterized by subcloning, restriction site mapping, and transposon Tn5 and Tn7 mutageneses. Three dct loci were identified within a 5.5-kilobase region of DNA, in the order dctA-dctB-dctC. The results suggested that dctA encoded a structural component necessary for C4-dicarboxylate transport, whereas dctB and dctC encoded positive regulatory elements, and that dctA was transcribed divergently from dctB and dctC. Expression of dctA and dctC was obtained from vector promoters in some pLAFR1- and pSUP106-based plasmids.

MeSH Terms
Biological Transport Carrier Proteins/genetics,metabolism Cloning, Molecular DNA Restriction Enzymes Dicarboxylic Acid Transporters Dicarboxylic Acids/metabolism Escherichia coli/genetics Genes Genes, Bacterial Genetic Complementation Test Genetic Vectors Mutation Plasmids Rhizobium/genetics,metabolism
Chemicals
Carrier Proteins Dicarboxylic Acid Transporters Dicarboxylic Acids DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ronson C W
Astwood P M
Downie J A
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22 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.160.3.903-909.1984
Published
1984-12-00
Pages
903-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215795
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.160.3.903-909.1984 PMC Full Text View journal details
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