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PMID: 3029432 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Proteolysis of the p220 component of the cap-binding protein complex is not sufficient for complete inhibition of host cell protein synthesis after poliovirus infection.

Journal of virology ·Vol. 61 ·No. 4 ·1987-04-00 ·Pages 986-91

Bonneau AM, Sonenberg N

Abstract

Infection of cells with poliovirus results in the complete shutoff of host protein synthesis. It is presumed that proteolysis of the p220 component of the cap-binding protein complex that is required for the translation of host mRNAs is responsible for the shutoff phenomenon. In this paper, we show that when cells are infected with poliovirus in the presence of guanidine or 3-methylquercetin, both inhibitors of poliovirus replication, complete cleavage of p220 occurs by 3.5 h postinfection. However, under these conditions only 55 to 77% of host protein synthesis is suppressed. Results obtained with extracts prepared from poliovirus-infected cells were similar to those obtained in vivo. These results suggest that complete inhibition of host protein synthesis after poliovirus infection requires at least one event in addition to proteolysis of p220. Thus, proteolysis of p220 is probably necessary but not sufficient for total suppression of host protein synthesis after poliovirus infection.

MeSH Terms
Antiviral Agents/pharmacology Carrier Proteins/metabolism Cell Transformation, Viral Flavonols Guanidine Guanidines/pharmacology HeLa Cells/metabolism Humans Poliovirus/drug effects,genetics Protein Biosynthesis Quercetin/analogs & derivatives,pharmacology RNA Cap-Binding Proteins RNA Caps/metabolism RNA, Messenger/genetics Transcription, Genetic
Chemicals
Antiviral Agents Carrier Proteins Flavonols Guanidines RNA Cap-Binding Proteins RNA Caps RNA, Messenger 3-methylquercetin Quercetin Guanidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bonneau A M
Sonenberg N
References (42)
42 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.4.986-991.1987
Published
1987-04-00
Pages
986-91
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254054
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.61.4.986-991.1987 PMC Full Text View journal details
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