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PMID: 269388 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

5'-Terminal 7-methylguanosine and mRNA function: influence of potassium concentration on translation in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 8 ·1977-08-00 ·Pages 3254-8

Weber LA, Hickey ED, Nuss DL, Baglioni C

Abstract

Vaccinia mRNAs containing either 5'-terminal m7G or unmethylated 5'-terminal structures were synthesized in vitro and their relative efficiencies of translation were compared in wheat germ and reticulocyte cell-free protein-synthesizing systems. The importance of the m7G group for efficient translation increases as the K+ concentration is raised. At K+ concentrations optimal for translation of mRNA containing m7G, unmethylated mRNA is translated at the same relative low efficiency in both cell extracts. The rate of binding of mRNA to ribosomes at K+ concentrations close to those found in intact cells is strongly influenced by the presence of m7G regardless of the source of the cell extract.

MeSH Terms
Animals Guanosine/analogs & derivatives,metabolism Kinetics Methylation Molecular Weight Plants/metabolism Poly A/metabolism Potassium/pharmacology Protein Biosynthesis/drug effects RNA, Messenger/metabolism Reticulocytes/metabolism Ribosomes/drug effects,metabolism Triticum/metabolism Vaccinia virus/metabolism
Chemicals
RNA, Messenger Guanosine Poly A Potassium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Weber L A
Hickey E D
Nuss D L
Baglioni C
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33 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.8.3254
Published
1977-08-00
Pages
3254-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431520
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.8.3254 PMC Full Text View journal details
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