Home LiteratureArticle Details
PMID: 14582204 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Capped mRNAs with reduced secondary structure can function in extracts from poliovirus-infected cells.

Molecular and cellular biology ·Vol. 2 ·No. 12 ·1982-12-00 ·Pages 1633-8

Sonenberg N, Guertin D, Lee KA

Abstract

Extracts from poliovirus-infected HeLa cells were used to study ribosome binding of native and denatured reovirus mRNAs and translation of capped mRNAs with different degrees of secondary structure. Here, we demonstrate that ribosomes in extracts from poliovirus-infected cells could form initiation complexes with denatured reovirus mRNA, in contrast to their inability to bind native reovirus mRNA. Furthermore, the capped alfalfa mosaic virus 4 RNA, which is most probably devoid of stable secondary structure at its 5' end, could be translated at much higher efficiency than could other capped mRNAs in extracts from poliovirus-infected cells.

MeSH Terms
HeLa Cells Humans Nucleic Acid Conformation Poliovirus/genetics,physiology Protein Biosynthesis RNA Caps/chemistry,metabolism
Chemicals
RNA Caps
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sonenberg N
Department of Biochemistry, McGill University, Montreal, Quebec, Canada H3G 1Y6.
Guertin D
Lee K A
References (38)
38 references, click to expand
  1. Nucleotide sequence of 5' terminus of alfalfa mosaic virus RNA 4 leading into coat protein cistron.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5504-8 PMID: 271973
  2. Influence of potassium salt concentration and temperature on inhibition of mRNA translation by 7-methylguanosine5'-monophosphate.
    J Biol Chem. 1978 Jan 10;253(1):178-83 PMID: 201617
  3. Translational recognition of the 5'-terminal 7-methylguanosine of globin messenger RNA as a function of ionic strength.
    Biochemistry. 1978 Jun 13;17(12):2450-5 PMID: 209816
  4. Inhibition of translation by poliovirus: inactivation of a specific initiation factor.
    Proc Natl Acad Sci U S A. 1978 Jun;75(6):2732-6 PMID: 208073
  5. A polypeptide in eukaryotic initiation factors that crosslinks specifically to the 5'-terminal cap in mRNA.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4843-7 PMID: 217002
  6. Ribosome-protected fragments from sindbis 42-S and 26-S RNAs.
    Eur J Biochem. 1979 Feb 15;94(1):41-50 PMID: 436845
  7. Competitive mRNA translation in an in vitro system from wheat germ.
    J Biol Chem. 1979 Sep 10;254(17):8245-9 PMID: 468823
  8. Eukaryotic mRNA cap binding protein: purification by affinity chromatography on sepharose-coupled m7GDP.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4345-9 PMID: 291969
  9. Influence of mRNA secondary structure on binding and migration of 40S ribosomal subunits.
    Cell. 1980 Jan;19(1):79-90 PMID: 7357609
  10. Secondary structure of mouse and rabbit alpha- and beta-globin mRNAs: differential accessibility of alpha and beta initiator AUG codons towards nucleases.
    Cell. 1980 Jan;19(1):91-102 PMID: 7357610
  11. Purification of a factor that restores translation of vesicular stomatitis virus mRNA in extracts from poliovirus-infected HeLa cells.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):770-4 PMID: 6244584
  12. 5'-terminal cap structure in eucaryotic messenger ribonucleic acids.
    Microbiol Rev. 1980 Jun;44(2):175-205 PMID: 6247631
  13. Restricted initiation of protein synthesis on the potentially polycistronic Sindbis virus 42 S RNA.
    J Biol Chem. 1980 Dec 10;255(23):11473-7 PMID: 7440550
  14. Role of ATP in binding and migration of 40S ribosomal subunits.
    Cell. 1980 Nov;22(2 Pt 2):459-67 PMID: 7448869
  15. Initiation of reovirus transcription by inosine 5'-triphosphate and properties of 7-methylinosine-capped, inosine-substituted messenger ribonucleic acids.
    Biochemistry. 1980 Dec 23;19(26):5960-6 PMID: 7470442
  16. ATP/Mg++-dependent cross-linking of cap binding proteins to the 5' end of eukaryotic mRNA.
    Nucleic Acids Res. 1981 Apr 10;9(7):1643-56 PMID: 7232223
  17. Presence of the cap-binding protein in initiation factor preparations from poliovirus-infected HeLa cells.
    J Virol. 1981 May;38(2):438-45 PMID: 6264121
  18. Two forms of purified m7G-cap binding protein with different effects on capped mRNA translation in extracts of uninfected and poliovirus-infected HeLa cells.
    J Biol Chem. 1981 Aug 10;256(15):7691-4 PMID: 6267020
  19. Computer-aided prediction of RNA secondary structures.
    Nucleic Acids Res. 1982 Jan 11;10(1):403-19 PMID: 6174937
  20. Characterization of eukaryotic initiation factor 4A, a protein involved in ATP-dependent binding of globin mRNA.
    J Biol Chem. 1982 May 10;257(9):5246-52 PMID: 7068683
  21. Poliovirus-induced inhibition of host-cell protein synthesis.
    Cell. 1982 Mar;28(3):435-6 PMID: 6280869
  22. Association of cap-binding protein with eucaryotic initiation factor 3 in initiation factor preparations from uninfected and poliovirus-infected HeLa cells.
    J Virol. 1982 Apr;42(1):200-7 PMID: 6283140
  23. Inactivation of cap-binding proteins accompanies the shut-off of host protein synthesis by poliovirus.
    Proc Natl Acad Sci U S A. 1982 Jun;79(11):3447-51 PMID: 6954488
  24. Probing the function of the eucaryotic 5' cap structure by using a monoclonal antibody directed against cap-binding proteins.
    Cell. 1981 Dec;27(3 Pt 2):563-72 PMID: 6101207
  25. Terminal heterogeneity at both ends of the satellite tobacco necrosis virus ribonucleic acid.
    Biochemistry. 1971 Dec 7;10(25):4748-52 PMID: 5140189
  26. In vitro translation of cardiovirus ribonucleic acid by mammalian cell-free extracts.
    J Virol. 1972 Apr;9(4):636-45 PMID: 4336562
  27. Improved methods for purification and assay of eukaryotic messenger ribonucleic acids and ribosomes. Quantitative analysis of their interaction in a fractionated reticulocyte cell-free system.
    J Biol Chem. 1975 Aug 10;250(15):6077-84 PMID: 1150672
  28. Determination of secondary structure in rabbit globin messenger RNA by thermal denaturation.
    Biochemistry. 1975 Sep 23;14(19):4209-15 PMID: 241373
  29. 5'-terminal structure of poliovirus polyribosomal RNA is pUp.
    Proc Natl Acad Sci U S A. 1976 Feb;73(2):327-30 PMID: 174102
  30. The 5' end of poliovirus mRNA is not capped with m7G(5')ppp(5')Np.
    Proc Natl Acad Sci U S A. 1976 Feb;73(2):375-80 PMID: 174106
  31. Translation in vitro of vesicular stomatitis virus mRNA lacking 5'-terminal 7-methylguanosine.
    Nature. 1976 Jul 1;262(5563):32-7 PMID: 180425
  32. Effect of 5'-terminal structure and base composition on polyribonucleotide binding to ribosomes.
    J Mol Biol. 1976 Jul 5;104(3):637-58 PMID: 950671
  33. Influence of 5'-terminal m7G and 2'--O-methylated residues on messenger ribonucleic acid binding to ribosomes.
    Biochemistry. 1976 Dec 28;15(26):5761-8 PMID: 1009086
  34. Capping of eucaryotic mRNAs.
    Cell. 1976 Dec;9(4 PT 2):645-53 PMID: 1017010
  35. 5'-Terminal 7-methylguanosine and mRNA function: influence of potassium concentration on translation in vitro.
    Proc Natl Acad Sci U S A. 1977 Aug;74(8):3254-8 PMID: 269388
  36. Importance of initiation factor preparations in the translation of reovirus and globin mRNAs lacking a 5'-terminal 7-methylguanosine.
    J Biol Chem. 1977 Dec 10;252(23):8489-97 PMID: 925007
  37. Reovirus mRNA can be covalently crosslinked via the 5' cap to proteins in initiation complexes.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4288-92 PMID: 270673
  38. 5'-Terminal 7-methylguanosine and mRNA function. The effect of enzymatic decapping and of cap analogs on translation of tobacco-mosaic-virus RNA and globin mRNA in vitro.
    Eur J Biochem. 1978 Dec 1;92(1):69-80 PMID: 729595
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.2.12.1633-1638.1982
Published
1982-12-00
Pages
1633-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369971
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.2.12.1633-1638.1982 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2