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PMID: 3023845 Published · ppublish English Journal Article

Structure of the highly repeated, long interspersed DNA family (LINE or L1Rn) of the rat.

Molecular and cellular biology ·Vol. 6 ·No. 2 ·1986-02-00 ·Pages 411-24

D'Ambrosio E, Waitzkin SD, Witney FR, Salemme A, Furano AV

Abstract

We present the DNA sequence of a 6.7-kilobase member of the rat long interspersed repeated DNA family (LINE or L1Rn). This member (LINE 3) is flanked by a perfect 14-base-pair (bp) direct repeat and is a full-length, or close-to-full-length, member of this family. LINE 3 contains an approximately 100-bp A-rich right end, a number of long (greater than 400-bp) open reading frames, and a ca. 200-bp G + C-rich (ca. 60%) cluster near each terminus. Comparison of the LINE 3 sequence with the sequence of about one-half of another member, which we also present, as well as restriction enzyme analysis of the genomic copies of this family, indicates that in length and overall structure LINE 3 is quite typical of the 40,000 or so other genomic members of this family which would account for as much as 10% of the rat genome. Therefore, the rat LINE family is relatively homogeneous, which contrasts with the heterogeneous LINE families in primates and mice. Transcripts corresponding to the entire LINE sequence are abundant in the nuclear RNA of rat liver. The characteristics of the rat LINE family are discussed with respect to the possible function and evolution of this family of DNA sequences.

MeSH Terms
Animals Base Composition Cloning, Molecular DNA/genetics,isolation & purification DNA Restriction Enzymes Molecular Weight Plasmids Rats Repetitive Sequences, Nucleic Acid
Chemicals
DNA DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
D'Ambrosio E
Waitzkin S D
Witney F R
Salemme A
Furano A V
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.2.411-424.1986
Published
1986-02-00
Pages
411-24
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367530
Subset
IM
Databases
GENBANK
M13100, M13101
External Links
PubMed source DOI: 10.1128/mcb.6.2.411-424.1986 PMC Full Text View journal details
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