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PMID: 6296779 Published · ppublish English Journal Article

Ubiquitous transposon-like repeats B1 and B2 of the mouse genome: B2 sequencing.

Nucleic acids research ·Vol. 10 ·No. 23 ·1982-12-11 ·Pages 7461-75

Krayev AS, Markusheva TV, Kramerov DA, Ryskov AP, Skryabin KG, Bayev AA, Georgiev GP

Abstract

Mouse genome contains two major families of short interspersed repeats in more than 10(5) copies scattered throughout the whole genome. They are referred to as B1 and B2 sequences since they were first isolated from the genome library by means of a dsRNA-B probe /1/. In this work, two copies of the B2 family were sequenced and compared with the previously sequenced B1 repeat /2/. A B2 ubiquitous repeat is ca. 190 bp long. The members of the family deviate in 3-5% of nucleotides from the consensus sequence. B2 contains regions of homology to the RNA polymerase III split promoter and to 4.5S snRNA I. Both B1 and B2 contain regions which resemble junctions between exons and introns. In contrast to B1, B2 does not contain apparent homologies to papova viral replication origins and a human Alu sequence. One side of the B2 repeat is represented by a very AT-rich sequence (ca. 30 bp long) followed with an oligo (dA) stretch 10-15 nucleotides long. This region of the repeat is the most variable one. The whole unit is flanked with 15-16 bp direct repeats different in sequenced copies of B2. The same is true of some copies of the B1 family. The properties of B1 and B2 repeats suggest that they may represent a novel class of transposon-like elements in eukaryotic genome. A possible role of B-type repeats in genome reorganization, DNA replication and pre-mRNA processing is discussed.

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA/genetics DNA Replication DNA Transposable Elements Genes Mice Nucleic Acid Hybridization Repetitive Sequences, Nucleic Acid
Chemicals
DNA Transposable Elements DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Krayev A S
Markusheva T V
Kramerov D A
Ryskov A P
Skryabin K G
Bayev A A
Georgiev G P
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.23.7461
Published
1982-12-11
Pages
7461-75
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327023
Subset
IM
Databases
GENBANK
K00131, K00132
External Links
PubMed source DOI: 10.1093/nar/10.23.7461 PMC Full Text View journal details
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