Long double-stranded sequences (dsRNA-B) of nuclear pre-mRNA consist of a few highly abundant classes of sequences: evidence from DNA cloning experiments.

Nucleic acids research ·Vol. 6 ·No. 2 ·1979-02-00 ·Pages 697-713

Kramerov DA, Grigoryan AA, Ryskov AP, Georgiev GP

Abstract

DNA preparations from about hundred randomly selected clones containing mouse DNA fragments were screened for the existence of sequences complementary to long double-stranded regions of pre-mRNA able to snap back after melting (dsRNA-B). Many clones containing such sequences were found. The cloned sequences can be subdivided into three groups: (1) those complementary to about a half (at least to 30-40%) of the total dsRNA, designated as sequences B1; (2) those complementary to a part of sequence B1; and (3) sequences complementary to about a quarter (at least to 15%) of the total dsRNA referred to as sequence B2. The size of DNA sequence complementary to dsRNA is about 400 base pairs. Melting experiments with hybrids show that the members of B1 family are very similar if not identical, while the divergence among B2 sequences is higher, but still the number of substitutions does not exceed 9% of bases. Thus, the major part of dsRNA-B consists of a small number of highly abundant sequences as was suggested earlier on the basis of renaturation kinetics /1-3/. Sequences B1 and B2 are represented by many copies in the mouse genome and in pre-mRNA, and many of them probably do not form hairpin-like structures.

MeSH Terms

Animals Base Sequence Carcinoma, Ehrlich Tumor/metabolism Cell Nucleus/metabolism DNA, Recombinant/metabolism Escherichia coli/metabolism Mice Nucleic Acid Denaturation Nucleic Acid Hybridization Plasmids RNA, Messenger/biosynthesis RNA, Neoplasm/metabolism

Chemicals

DNA, Recombinant RNA, Messenger RNA, Neoplasm

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Kramerov D A

Grigoryan A A

Ryskov A P

Georgiev G P

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22 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/6.2.697

Published

1979-02-00

Pages

697-713

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327722

Subset

External Links

PubMed source DOI: 10.1093/nar/6.2.697 PMC Full Text View journal details

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Long double-stranded sequences (dsRNA-B) of nuclear pre-mRNA consist of a few highly abundant classes of sequences: evidence from DNA cloning experiments.

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