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PMID: 4506771 Published · ppublish English Journal Article

Double-stranded regions in heterogeneous nuclear RNA from Hela cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 9 ·1972-09-00 ·Pages 2537-41

Jelinek W, Darnell JE

Abstract

Heterogeneous nuclear RNA from HeLa cells contains double-stranded regions that arise by base pairing of complementary sequences that exist as parts of the same molecule (intramolecular base pairing). When denatured, the RNA sequences that form the double-stranded regions hybridize rapidly to HeLa cell DNA, suggesting that they are transcribed from reiterated sites in the genome. The messenger RNA does not contain the same class or amount of double-stranded RNA regions found in heterogeneous nuclear RNA.

MeSH Terms
Cell Nucleus/analysis Centrifugation, Density Gradient Chemical Precipitation Chromatography Coliphages/enzymology Electrophoresis HeLa Cells/analysis,cytology Hydroxyapatites Lithium Nucleic Acid Denaturation Nucleic Acid Hybridization Pancreas/enzymology Phosphorus Isotopes RNA, Messenger/analysis RNA, Neoplasm/analysis,isolation & purification Ribonucleases Tritium
Chemicals
Hydroxyapatites Phosphorus Isotopes RNA, Messenger RNA, Neoplasm Tritium Lithium Ribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jelinek W
Darnell J E
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.9.2537
Published
1972-09-00
Pages
2537-41
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC426983
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.9.2537 PMC Full Text View journal details
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