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PMID: 7232213 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structural analysis of interspersed repetitive polymerase III transcription units in human DNA.

Nucleic acids research ·Vol. 9 ·No. 5 ·1981-03-11 ·Pages 1151-70

Pan J, Elder JT, Duncan CH, Weissman SM

Abstract

The nucleotide sequences of two cloned fragments of human DNA which function as templates for RNA polymerase III in vitro confirm their identities as members of the Alu family of human interspersed repetitive DNA sequences (1,2). The interspersed and repetitive nature of these sequences in the genome was demonstrated by hybridization of nick-translated DNA from one of these clones to total genomic DNA and to DNA of individual random clones from a lambda Ch4A-based human genomic library. Short, direct terminal repeats of non-conserved sequence flank the 300 nucleotide Alu family conserved sequence. Within the Alu family sequence is found a 40-nucleotide region which is directly repeated 135 nucleotides downstream. This 40 nucleotide sequence is found once in the murine B1 interspersed repetitive sequence family (8). This and other evidence indicates that the human Alu family resembles a partial duplication of the murine B1 sequence.

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA/analysis DNA-Directed RNA Polymerases/metabolism Humans Mice Nucleic Acid Hybridization RNA Polymerase III/metabolism Repetitive Sequences, Nucleic Acid Species Specificity Templates, Genetic Transcription, Genetic
Chemicals
DNA DNA-Directed RNA Polymerases RNA Polymerase III
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pan J
Elder J T
Duncan C H
Weissman S M
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33 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
Published
1981-03-11
Pages
1151-70
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326743
Subset
IM
Grants
NCI NIH HHS · 2 POL CA 16038-06 · United States
NCI NIH HHS · CA 16359 · United States
Databases
GENBANK
J00095
External Links
PubMed source PMC Full Text View journal details
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