Kpn I family of long interspersed repeated DNA sequences in primates: polymorphism of family members and evidence for transcription.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 13 ·1983-07-00 ·Pages 3966-70

Lerman MI, Thayer RE, Singer MF

Abstract

An approximately equal to 2-kilobase-pair-long member (Kpn I-LS1) of the African green monkey Kpn I family of repeated sequences has been cloned, subjected to sequence analysis, and compared to other family members which are over 6 kilobase pairs (Kpn I-alpha 7) and 829 base pairs (Kpn I-RET) long. Both Kpn I-LS1 and Kpn I-RET lack sequences found at the ends of the longer family member and their structures resemble those of processed genes. Kpm I-LS1 sequences are colinear with part of the long family member, Kpn I-alpha 7. However, although all sequences in Kpn I-RET are represented in Kpn I-LS1, the two are not colinear; Kpn I-RET is missing 731 base pairs found in Kpn I-LS1 and one segment flanking the deletion is inverted. The results demonstrate that Kpn I family members are not only of different lengths but may also contain scrambled arrangements of common sequences. Sequences in Kpn I-LS1 hybridize to RNA from monkey and human cells, indicating that some family members are transcribed.

MeSH Terms

Animals Base Composition Chlorocebus aethiops Cloning, Molecular DNA Restriction Enzymes Genes Nucleic Acid Hybridization Polymorphism, Genetic Repetitive Sequences, Nucleic Acid Transcription, Genetic

Chemicals

DNA Restriction Enzymes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Lerman M I

Thayer R E

Singer M F

References (38)

38 references, click to expand

DNA strand reassociation and polyribonucleotide binding in the African green monkey, Cercopithecus aethiops.
J Mol Biol. 1971 Mar 28;56(3):579-95 PMID: 4995480
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
Ribonucleic acid isolated by cesium chloride centrifugation.
Biochemistry. 1974 Jun 4;13(12):2633-7 PMID: 4831907
Inversion of the G DNA segment of phage Mu controls phage infectivity.
Nature. 1978 Feb 9;271(5645):577-80 PMID: 622196
MspI, an isoschizomer of hpaII which cleaves both unmethylated and methylated hpaII sites.
Nucleic Acids Res. 1978 Sep;5(9):3231-6 PMID: 704354
The isolation of structural genes from libraries of eucaryotic DNA.
Cell. 1978 Oct;15(2):687-701 PMID: 719759
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
The clustered and scrambled arrangement of moderately repetitive elements in Drosophila DNA.
Cell. 1979 Dec;18(4):1231-46 PMID: 117904
An improved method for detecting foreign DNA in plasmids of Escherichia coli.
Anal Biochem. 1979 Sep 15;98(1):60-3 PMID: 120689
Sequencing end-labeled DNA with base-specific chemical cleavages.
Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
Computer analysis of nucleic acids and proteins.
Methods Enzymol. 1980;65(1):595-609 PMID: 6154871
Phase variation: evolution of a controlling element.
Science. 1980 Sep 19;209(4463):1370-4 PMID: 6251543
Nucleotide sequence of the E coli gene coding for dihydrofolate reductase.
Nucleic Acids Res. 1980 May 24;8(10):2255-74 PMID: 6159575
Reiteration frequency mapping: analysis of repetitive sequence organization within cloned DNA fragments containing the human initiator methionine tRNA gene.
Proc Natl Acad Sci U S A. 1980 Oct;77(10):5668-72 PMID: 6934502
A family of long reiterated DNA sequences, one copy of which is next to the human beta globin gene.
Nucleic Acids Res. 1980 Dec 20;8(24):6113-28 PMID: 6258162
DNA sequences similar to those around the simian virus 40 origin of replication are present in the monkey genome.
Proc Natl Acad Sci U S A. 1981 Jan;78(1):95-9 PMID: 6264457
Interspersed repeated sequences in the African green monkey genome that are homologous to the human Alu family.
Nucleic Acids Res. 1981 Nov 11;9(21):5553-68 PMID: 6273798
Direct repeats flank three small nuclear RNA pseudogenes in the human genome.
Cell. 1981 Oct;26(1 Pt 1):11-7 PMID: 6173130
Base sequence studies of 300 nucleotide renatured repeated human DNA clones.
J Mol Biol. 1981 Sep 5;151(1):17-33 PMID: 6276559
Short interspersed repetitive DNA elements in eucaryotes: transposable DNA elements generated by reverse transcription of RNA pol III transcripts?
Cell. 1981 Oct;26(2 Pt 2):141-2 PMID: 6277493
Nucleotide sequence of 7 S RNA. Homology to Alu DNA and La 4.5 S RNA.
J Biol Chem. 1982 May 10;257(9):5136-42 PMID: 6802847
Structural features of the DNA flanking the human non-alpha globin genes: implications in the control of fetal hemoglobin switching.
Trans Assoc Am Physicians. 1981;94:204-10 PMID: 6178203
KpnI families of long, interspersed repetitive DNAs in human and other primate genomes.
Nucleic Acids Res. 1982 May 25;10(10):3175-93 PMID: 6285290
Nucleotide sequence definition of a major human repeated DNA, the Hind III 1.9 kb family.
Nucleic Acids Res. 1982 May 25;10(10):3211-9 PMID: 6285292
Genomic representation of the Hind II 1.9 kb repeated DNA.
Nucleic Acids Res. 1982 May 25;10(10):3221-39 PMID: 6285293
Human 7SL RNA consists of a 140 nucleotide middle-repetitive sequence inserted in an alu sequence.
Cell. 1982 May;29(1):195-202 PMID: 6179628
Three segments from the monkey genome that hybridize to simian virus 40 have common structural elements.
Mol Cell Biol. 1981 Dec;1(12):1061-8 PMID: 6287216
Joining of immunoglobulin heavy chain gene segments: implications from a chromosome with evidence of three D-JH fusions.
Proc Natl Acad Sci U S A. 1982 Jul;79(13):4118-22 PMID: 6287467
Repetitive sequences in eukaryotic DNA and their expression.
Annu Rev Biochem. 1982;51:813-44 PMID: 7051966
Highly repeated sequences in mammalian genomes.
Int Rev Cytol. 1982;76:67-112 PMID: 6749748
Synthesis in vitro of an exceptionally long RNA transcript promoted by an AluI sequence.
Nature. 1982 Nov 25;300(5890):376-9 PMID: 7144892
A switch region inversion contributes to the aberrant rearrangement of a mu immunoglobulin heavy chain gene in MPC-11 cells.
Nucleic Acids Res. 1982 Dec 11;10(23):7751-61 PMID: 6296790
Members of the KpnI family of long interspersed repeated sequences join and interrupt alpha-satellite in the monkey genome.
Nucleic Acids Res. 1983 Jan 25;11(2):321-38 PMID: 6298721
Rapid similarity searches of nucleic acid and protein data banks.
Proc Natl Acad Sci U S A. 1983 Feb;80(3):726-30 PMID: 6572363
KpnI families of long, interspersed repetitive DNAs associated with the human beta-globin gene cluster.
Gene. 1982 Dec;20(3):397-407 PMID: 6299899
A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells.
Mol Cell Biol. 1983 Feb;3(2):280-9 PMID: 6300662
Unusual class of Alu sequences containing a potential Z-DNA segment.
Mol Cell Biol. 1983 May;3(5):960-4 PMID: 6865948
Human U1 RNA pseudogenes may be generated by both DNA- and RNA-mediated mechanisms.
Mol Cell Biol. 1982 Jul;2(7):815-28 PMID: 6085956

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.80.13.3966

Published

1983-07-00

Pages

3966-70

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC394180

Subset

Databases

GENBANK

J00337, J00338

External Links

PubMed source DOI: 10.1073/pnas.80.13.3966 PMC Full Text View journal details

Analysis Services

Kpn I family of long interspersed repeated DNA sequences in primates: polymorphism of family members and evidence for transcription.

Contact

WeChat Official Account