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PMID: 3023684 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Construction and characterization of hybrid polyomavirus genomes.

Journal of virology ·Vol. 60 ·No. 3 ·1986-12-00 ·Pages 960-71

Chuke WF, Walker DL, Peitzman LB, Frisque RJ

Abstract

Several studies have suggested that certain unique features of the JC virus (JCV) regulatory region are responsible for the restricted lytic and transforming activities of this virus in vitro. To pursue this possibility, we have constructed hybrid polyomavirus genomes by exchanging the regulatory sequences of JCV, BK virus (BKV), and simian virus 40 (SV40). The host range of JCV was not expanded by the substitution of the BKV or SV40 regulatory signals; such hybrids were nonviable even in primary human fetal glial cells, the sole permissive cell for JCV. However, chimeric DNAs containing JCV regulatory sequences and BKV- or SV40-coding sequences were lytically active, indicating that the BKV and SV40 T proteins were capable of effectively interacting with the JCV replication and transcription signals to yield infectious hybrid viruses. Although JCV regulatory sequences and coding sequences both contributed to the restricted lytic activity of this virus, it appears that the latter sequences, most likely hose encoding the T protein, have a greater influence on this behavior.

MeSH Terms
Antigens, Viral, Tumor/genetics Base Sequence Cell Line DNA, Recombinant DNA, Viral/genetics Gene Expression Regulation Genes Genes, Regulator Genes, Viral Polyomavirus/genetics Transfection Viral Proteins/genetics Virus Replication
Chemicals
Antigens, Viral, Tumor DNA, Recombinant DNA, Viral Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chuke W F
Walker D L
Peitzman L B
Frisque R J
References (85)
85 references, click to expand
  1. Characterization of enhancer elements in the long terminal repeat of Moloney murine sarcoma virus.
    J Virol. 1984 Jan;49(1):183-9 PMID: 6690710
  2. DNA-binding properties of simian virus 40 T-antigen mutants defective in viral DNA replication.
    Mol Cell Biol. 1983 Nov;3(11):1958-66 PMID: 6318076
  3. Specific interaction between enhancer-containing molecules and cellular components.
    Cell. 1984 Feb;36(2):403-11 PMID: 6319021
  4. Simian virus 40 mutant T antigens with relaxed specificity for the nucleotide sequence at the viral DNA origin of replication.
    J Virol. 1984 Feb;49(2):386-93 PMID: 6319739
  5. An enhancer element is located 340 base pairs upstream from the adenovirus-2 E1A capsite.
    Nucleic Acids Res. 1983 Dec 20;11(24):8747-60 PMID: 6324099
  6. Simian virus 40 large T-antigen point mutants that are defective in viral DNA replication but competent in oncogenic transformation.
    Mol Cell Biol. 1984 Jun;4(6):1125-33 PMID: 6330530
  7. Human polyomavirus JC virus genome.
    J Virol. 1984 Aug;51(2):458-69 PMID: 6086957
  8. A 3' end fragment encompassing the transcriptional enhancers of nondefective Friend virus confers erythroleukemogenicity on Moloney leukemia virus.
    J Virol. 1984 Oct;52(1):248-54 PMID: 6090701
  9. Promoter dependence of enhancer activity.
    Mol Cell Biol. 1984 Aug;4(8):1664-8 PMID: 6092930
  10. Activation of SV40 genome by 72-base pair tandem repeats of Moloney sarcoma virus.
    Nature. 1982 Feb 18;295(5850):568-72 PMID: 6276775
  11. Expression of a beta-globin gene is enhanced by remote SV40 DNA sequences.
    Cell. 1981 Dec;27(2 Pt 1):299-308 PMID: 6277502
  12. Efficient infection of monkey cells with DNA of simian virus 40.
    Proc Natl Acad Sci U S A. 1981 Dec;78(12):7575-8 PMID: 6278479
  13. Mutant of simian virus 40 large T-antigen that is defective for viral DNA synthesis, but competent for transformation of cultured rat cells.
    J Virol. 1982 Jun;42(3):854-64 PMID: 6285001
  14. Change of DNA near the origin of replication enhances the transforming capacity of human papovavirus BK.
    J Virol. 1982 Jun;42(3):978-85 PMID: 6285007
  15. Is progressive multifocal leukoencephalopathy a chronic disease because of defective interfering particles or temperature-sensitive mutants of JC virus?
    J Virol. 1982 Sep;43(3):1143-50 PMID: 6292458
  16. Host-specific activation of transcription by tandem repeats from simian virus 40 and Moloney murine sarcoma virus.
    Proc Natl Acad Sci U S A. 1982 Nov;79(21):6453-7 PMID: 6292901
  17. SV40 early mutants that are defective for viral DNA synthesis but competent for transformation of cultured rat and simian cells.
    Virology. 1982 Nov;123(1):78-92 PMID: 6293195
  18. Multiple point mutations affecting the simian virus 40 enhancer.
    Science. 1983 Feb 11;219(4585):626-31 PMID: 6297005
  19. Topography of simian virus 40 A protein-DNA complexes: arrangement of pentanucleotide interaction sites at the origin of replication.
    J Virol. 1983 Apr;46(1):143-50 PMID: 6298451
  20. Nucleotide sequence of the region encompassing the JC virus origin of DNA replication.
    J Virol. 1983 Apr;46(1):170-6 PMID: 6298454
  21. Definition of the simian virus 40 early promoter region and demonstration of a host range bias in the enhancement effect of the simian virus 40 72-base-pair repeat.
    Proc Natl Acad Sci U S A. 1983 Feb;80(3):721-5 PMID: 6298771
  22. Binding of SV40 a protein to the BK virus origin of DNA replication.
    Virology. 1983 Aug;129(1):239-45 PMID: 6310883
  23. Characterization of tissue culture-induced heterogeneity in DNAs of independent isolates of JC virus.
    J Gen Virol. 1983 Oct;64 (Pt 10):2271-80 PMID: 6311956
  24. In vitro mutagenesis of a putative DNA binding domain of SV40 large-T.
    Virology. 1984 Nov;139(1):109-37 PMID: 6093377
  25. Tissue-specific transcription preference as a determinant of cell tropism and leukaemogenic potential of murine retroviruses.
    Nature. 1984 Nov 8-14;312(5990):159-62 PMID: 6095084
  26. JC virus enhancer-promoter active in human brain cells.
    Science. 1984 Dec 14;226(4680):1337-9 PMID: 6095453
  27. Two distinct enhancers with different cell specificities coexist in the regulatory region of polyoma.
    Cell. 1984 Dec;39(3 Pt 2):653-62 PMID: 6096017
  28. Genetic and biochemical analysis of transformation-competent, replication-defective simian virus 40 large T antigen mutants.
    J Virol. 1985 Jan;53(1):120-7 PMID: 2981330
  29. Differences in regulatory sequences of naturally occurring JC virus variants.
    J Virol. 1985 Jan;53(1):306-11 PMID: 2981353
  30. Two separable functional domains of simian virus 40 large T antigen: carboxyl-terminal region of simian virus 40 large T antigen is required for efficient capsid protein synthesis.
    J Virol. 1985 Feb;53(2):415-24 PMID: 2982029
  31. Mutations near the carboxyl terminus of the simian virus 40 large tumor antigen alter viral host range.
    J Virol. 1985 May;54(2):569-75 PMID: 2985819
  32. Tissue selectivity of murine leukemia virus infection is determined by long terminal repeat sequences.
    J Virol. 1985 Sep;55(3):862-6 PMID: 2991605
  33. SV40 enhancer and large-T antigen are instrumental in development of choroid plexus tumours in transgenic mice.
    Nature. 1985 Aug 1-7;316(6027):457-60 PMID: 2991771
  34. Simian virus 40 DNA replication in vitro: specificity of initiation and evidence for bidirectional replication.
    Mol Cell Biol. 1985 Jun;5(6):1238-46 PMID: 2993858
  35. Host species specificity of polyomavirus DNA replication is not altered by simian virus 40 72-base-pair repeats.
    Mol Cell Biol. 1985 Jun;5(6):1534-7 PMID: 2993870
  36. Simian virus 40 origin DNA-binding domain on large T antigen.
    J Virol. 1986 Jan;57(1):50-64 PMID: 3001365
  37. Properties of the simian virus 40 (SV40) large T antigens encoded by SV40 mutants with deletions in gene A.
    J Virol. 1986 Feb;57(2):539-46 PMID: 3003386
  38. Mapping 5' termini of JC virus early RNAs.
    J Virol. 1986 May;58(2):651-4 PMID: 3009875
  39. Characterization of cells transformed by the human polyomavirus JC virus.
    J Gen Virol. 1986 Aug;67 ( Pt 8):1733-9 PMID: 3016161
  40. Selective extraction of polyoma DNA from infected mouse cell cultures.
    J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
  41. Cultivation of papova-like virus from human brain with progressive multifocal leucoencephalopathy.
    Lancet. 1971 Jun 19;1(7712):1257-60 PMID: 4104715
  42. Prevalence of antibodies in human sera against JC virus, an isolate from a case of progressive multifocal leukoencephalopathy.
    J Infect Dis. 1973 Apr;127(4):467-70 PMID: 4571704
  43. The evolution of new species of viral DNA during serial passage of simian virus 40 at high multiplicity.
    Virology. 1973 Aug;54(2):384-97 PMID: 4353519
  44. Evolutionary variants of simian virus 40: cloned substituted variants containing multiple initiation sites for DNA replication.
    Virology. 1975 Jul;66(1):53-69 PMID: 166505
  45. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  46. Complementation between BK human papovavirus and a simian virus 40 tsA mutant.
    J Virol. 1976 Mar;17(3):1060-2 PMID: 176441
  47. Construction and analysis of viable deletion mutants of simian virus 40.
    J Virol. 1976 May;18(2):664-71 PMID: 178902
  48. JC Papovavirus in progressive multifocal leukoencephalopathy.
    J Infect Dis. 1976 Jun;133(6):686-90 PMID: 778304
  49. New human papovaviruses.
    Prog Med Virol. 1976;22:1-35 PMID: 62371
  50. JC virus, a human polyomavirus associated with progressive multifocal leukoencephalopathy: additional biological characteristics and antigenic relationships.
    Infect Immun. 1977 Feb;15(2):656-62 PMID: 191404
  51. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  52. Complete nucleotide sequence of SV40 DNA.
    Nature. 1978 May 11;273(5658):113-20 PMID: 205802
  53. The genome of simian virus 40.
    Science. 1978 May 5;200(4341):494-502 PMID: 205947
  54. Brain tumors in owl monkeys inoculated with a human polyomavirus (JC virus).
    Science. 1978 Sep 29;201(4362):1246-9 PMID: 211583
  55. The colinear alignment of the genomes of papovaviruses JC, BK, and SV40.
    Virology. 1979 Jul 30;96(2):576-87 PMID: 223318
  56. Helper function for adenovirus replication in monkey cells by BK human papovavirus.
    Virology. 1979 Oct 15;98(1):279-82 PMID: 225869
  57. Protein-DNA interactions at the origin of simian virus 40 DNA replication.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 2:655-61 PMID: 226310
  58. Infectivity of the DNA from four isolates of JC virus.
    J Virol. 1979 Nov;32(2):476-82 PMID: 228071
  59. BK virus DNA: complete nucleotide sequence of a human tumor virus.
    Science. 1979 Oct 26;206(4417):456-62 PMID: 228391
  60. Viable deletion mutant of human papovavirus BK that induces insulinomas in hamsters.
    J Virol. 1979 Dec;32(3):934-42 PMID: 229273
  61. The genome of human papovavirus BKV.
    Cell. 1979 Dec;18(4):963-77 PMID: 229976
  62. Experimental glioma in primates: a computed tomography model.
    J Comput Assist Tomogr. 1980 Jun;4(3):285-90 PMID: 6246149
  63. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  64. Expression of polyoma early functions in mouse embryonal carcinoma cells depends on sequence rearrangements in the beginning of the late region.
    Cell. 1980 Jun;20(2):393-9 PMID: 6248241
  65. Transformation of primary hamster brain cells with JC virus and its DNA.
    J Virol. 1980 Jul;35(1):265-9 PMID: 6251275
  66. Expression of early genes of origin-defective mutants of simian virus 40.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3898-902 PMID: 6254000
  67. Cloned human polyomavirus JC DNA can transform human amnion cells.
    J Virol. 1980 Dec;36(3):878-82 PMID: 6257931
  68. Effect of the host cell on the generation of defective Simian Virus 40 during undiluted serial passages and persistent infection.
    Virology. 1981 Jan 30;108(2):525-30 PMID: 6258302
  69. In vivo sequence requirements of the SV40 early promotor region.
    Nature. 1981 Mar 26;290(5804):304-10 PMID: 6259538
  70. Polyoma DNA sequences involved in control of viral gene expression in murine embryonal carcinoma cells.
    Nature. 1981 Apr 23;290(5808):720-2 PMID: 6261151
  71. Mutation near the polyoma DNA replication origin permits productive infection of F9 embryonal carcinoma cells.
    Cell. 1981 Mar;23(3):809-14 PMID: 6261957
  72. Identification of a promoter component involved in positioning the 5' termini of simian virus 40 early mRNAs.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):100-4 PMID: 6264425
  73. Amplification of papovavirus defectives during serial low multiplicity infections.
    Virology. 1981 Jul 30;112(2):800-3 PMID: 6266155
  74. The SV40 72 base repair repeat has a striking effect on gene expression both in SV40 and other chimeric recombinants.
    Nucleic Acids Res. 1981 Nov 25;9(22):6047-68 PMID: 6273820
  75. A small segment of polyoma virus DNA enhances the expression of a cloned beta-globin gene over a distance of 1400 base pairs.
    Nucleic Acids Res. 1981 Dec 11;9(23):6251-64 PMID: 6275354
  76. Mutational analysis of simian virus 40 T antigen: isolation and characterization of mutants with deletions in the T-antigen gene.
    Mol Cell Biol. 1983 Feb;3(2):203-13 PMID: 6300656
  77. Biochemical activities of T-antigen proteins encoded by simian virus 40 A gene deletion mutants.
    Mol Cell Biol. 1983 Feb;3(2):220-8 PMID: 6300658
  78. Enhanced transformation by a simian virus 40 recombinant virus containing a Harvey murine sarcoma virus long terminal repeat.
    Mol Cell Biol. 1983 Mar;3(3):325-39 PMID: 6302472
  79. Regulatory sequences and virus-cell interactions of JC virus.
    Prog Clin Biol Res. 1983;105:41-59 PMID: 6304771
  80. The adenovirus type 5 E1A transcriptional control region contains a duplicated enhancer element.
    Cell. 1983 Jul;33(3):695-703 PMID: 6871991
  81. A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene.
    Cell. 1983 Jul;33(3):717-28 PMID: 6409417
  82. A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes.
    Cell. 1983 Jul;33(3):729-40 PMID: 6409418
  83. The promoter-specific transcription factor Sp1 binds to upstream sequences in the SV40 early promoter.
    Cell. 1983 Nov;35(1):79-87 PMID: 6313230
  84. BK viral enhancer element and a human cellular homolog.
    Science. 1983 Nov 18;222(4625):749-55 PMID: 6314501
  85. Cell-specific expression controlled by the 5'-flanking region of insulin and chymotrypsin genes.
    Nature. 1983 Dec 8-14;306(5943):557-61 PMID: 6358900
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.60.3.960-971.1986
Published
1986-12-00
Pages
960-71
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253334
Subset
IM
Grants
NIAID NIH HHS · AI-11217 · United States
NCI NIH HHS · CA-38789 · United States
Databases
GENBANK
M14451, M14452
External Links
PubMed source DOI: 10.1128/JVI.60.3.960-971.1986 PMC Full Text View journal details
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