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PMID: 6285007 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Change of DNA near the origin of replication enhances the transforming capacity of human papovavirus BK.

Journal of virology ·Vol. 42 ·No. 3 ·1982-06-00 ·Pages 978-85

Watanabe S, Yoshiike K

Abstract

A turbid-plaque-forming mutant (pm522) of human papovavirus BK, which has a small deletion at about 0.7 map unit and grows somewhat more slowly in human cells than does wild-type BK virus, transformed hamster and rat cells in culture much more efficiently than did wild-type virus. Another plaque morphology mutant, pm525, forming turbid plaques larger than those of pm522 also had a high transforming capacity. The similar difference in transforming capability between wild-type and plaque morphology viruses was observed with DNAs extracted from virions. Recombinant viruses were constructed from the wild-type DNA fragment lacking HindIII-C (0.62 to 0.73 map unit) and pm522 HindIII-C (including the origin of replication) by the molecular cloning method. Characterization of the recombinants showed that the change near the origin of DNA replication was responsible both for the altered plaque morphology and for the enhanced transforming capacity of the BK virus mutant.

MeSH Terms
Animals BK Virus/genetics,physiology Cell Line Cell Transformation, Viral Cricetinae DNA Replication DNA Restriction Enzymes DNA, Recombinant DNA, Viral/physiology Deoxyribonuclease HindIII Genes, Viral Mutation Polyomavirus/physiology Rats Virus Replication
Chemicals
DNA, Recombinant DNA, Viral DNA Restriction Enzymes Deoxyribonuclease HindIII
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Watanabe S
Yoshiike K
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32 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.42.3.978-985.1982
Published
1982-06-00
Pages
978-85
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256931
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.42.3.978-985.1982 PMC Full Text View journal details
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