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PMID: 173887 Published · ppublish English Journal Article

Transformation of hamster kidney cells by BK papovavirus DNA.

Journal of virology ·Vol. 17 ·No. 1 ·1975-01-00 ·Pages 247-53

Takemoto KK, Martin MA

Abstract

Supercoiled BK papovavirus DNA was shown to transform hamster kidney cells using the calcium phosphate co-precipitation technique. The transformed cells contained intranuclear T-antigen(s) and rescuable virus and produced progressively growing tumors when inoculated into hamsters. A novel finding was the production in tumor-bearing animals of antinuclear antibody, which reacted against normal, untransformed cells; in addition, tumor serum contained antibody against virus-specific T-antigen(s).

MeSH Terms
Animals Antibodies, Antinuclear/analysis Antibodies, Neoplasm/analysis Antigens, Neoplasm/analysis Antigens, Viral/analysis BK Virus/growth & development,immunology Calcium Phosphates Cell Fusion Cell Line Cell Nucleus/immunology Cell Transformation, Neoplastic Chemical Precipitation Cricetinae DNA, Viral Kidney Neoplasms, Experimental/immunology Polyomavirus Virus Replication
Chemicals
Antibodies, Antinuclear Antibodies, Neoplasm Antigens, Neoplasm Antigens, Viral Calcium Phosphates DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Takemoto K K
Martin M A
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20 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.17.1.247-253.1976
Published
1975-01-00
Pages
247-53
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC515409
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.17.1.247-253.1976 PMC Full Text View journal details
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