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PMID: 3022012 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Production of guanidine-resistant and -dependent poliovirus mutants from cloned cDNA: mutations in polypeptide 2C are directly responsible for altered guanidine sensitivity.

Journal of virology ·Vol. 60 ·No. 2 ·1986-11-00 ·Pages 793-6

Pincus SE, Wimmer E

Abstract

cDNA fragments representing the region in polypeptide 2C containing mutations in a guanidine-resistant or -dependent mutant were cloned into the wild-type background of an infectious clone. Transfection of COS-1 cells with these plasmids yielded viruses that were either completely resistant to 2.0 mM guanidine hydrochloride or dependent on this concentration of drug for growth.

MeSH Terms
Cell Line Cloning, Molecular DNA Drug Resistance, Microbial Genes, Viral Guanidine Guanidines/pharmacology Mutation Peptides/genetics Poliovirus/drug effects,genetics Transfection Viral Proteins/genetics
Chemicals
Guanidines Peptides Viral Proteins DNA Guanidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pincus S E
Wimmer E
References (24)
24 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.60.2.793-796.1986
Published
1986-11-00
Pages
793-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC288960
Subset
IM
Grants
NIAID NIH HHS · AI-06920 · United States
NIAID NIH HHS · AI-15122 · United States
NCI NIH HHS · CA-28146 · United States
External Links
PubMed source DOI: 10.1128/JVI.60.2.793-796.1986 PMC Full Text View journal details
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