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PMID: 6283124 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Inhibition of poliovirus polymerase by guanidine in vitro.

Journal of virology ·Vol. 41 ·No. 1 ·1982-01-00 ·Pages 313-8

Tershak DR

Abstract

Extracts from poliovirus-infected HeLa cells synthesized 35s viral RNA, replicative intermediate, and double-stranded RNA in vitro. Guanidine inhibited the synthesis of all three species of RNA; production of 35s RNA was most sensitive to the inhibitor. Pulse-chase experiments with [3H]UTP indicated that guanidine had no detectable effect on elongation of polynucleotide chains or the release of completed RNA chains from the viral replication complex. Experiments in which short pulses of precursor were used suggest that guanidine blocked the initiation step of RNA synthesis in vitro.

MeSH Terms
DNA-Directed RNA Polymerases/antagonists & inhibitors Female Guanidines/pharmacology HeLa Cells Humans Poliovirus/enzymology RNA, Viral/biosynthesis
Chemicals
Guanidines RNA, Viral DNA-Directed RNA Polymerases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Tershak D R
References (28)
28 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.41.1.313-318.1982
Published
1982-01-00
Pages
313-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256753
Subset
IM
Grants
NIAID NIH HHS · AI16932 · United States
External Links
PubMed source DOI: 10.1128/JVI.41.1.313-318.1982 PMC Full Text View journal details
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