Home LiteratureArticle Details
PMID: 3003395 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Guanidine-selected mutants of poliovirus: mapping of point mutations to polypeptide 2C.

Journal of virology ·Vol. 57 ·No. 2 ·1986-02-00 ·Pages 638-46

Pincus SE, Diamond DC, Emini EA, Wimmer E

Abstract

Sequence analysis of the genomic RNA of interstrain guanidine-resistant and antibody-resistant variant recombinants of poliovirus type 1 mapped the resistance of mutants capable of growth in 2.0 mM guanidine hydrochloride to a region located 3' of nucleotide 4444. This region of the viral genome specifies the nonstructural protein 2C. The sequence of genomic RNA encoding 2C from six independently isolated mutants resistant to 2.0 mM guanidine was determined. All six isolates contained a mutation in 2C at the same position in all cases, resulting in two types of amino acid changes. Dependent mutants were examined and found to contain two amino acid changes each within 2C. Mutants resistant to 0.53 mM guanidine were isolated and found to lack the mutations seen in variants resistant to 2.0 mM guanidine. A comparison of the amino acid sequences of the 2C proteins of poliovirus, foot-and-mouth disease virus, rhinovirus types 2 and 14, and encephalomyocarditis virus revealed a strong homology over regions totaling 115 residues. All of the mutations observed in guanidine-selected mutants were contained within this region. The amino acid region containing the mutations observed in poliovirus mutants resistant to 2.0 mM guanidine was compared with the homologous region in the other picornaviruses; a strong correlation was found between the amino acid present at this position and the sensitivity of the virus to 2.0 mM guanidine.

MeSH Terms
Amino Acid Sequence Base Sequence Chromosome Mapping Dose-Response Relationship, Drug Drug Resistance, Microbial Encephalomyocarditis virus/genetics Guanidine Guanidines/pharmacology Poliovirus/drug effects,genetics RNA, Viral/genetics Selection, Genetic Sequence Homology, Nucleic Acid Viral Proteins/genetics Virus Replication/drug effects
Chemicals
Guanidines RNA, Viral Viral Proteins Guanidine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pincus S E
Diamond D C
Emini E A
Wimmer E
References (49)
49 references, click to expand
  1. Guanidine-resistant poliovirus mutants produce modified 37-kilodalton proteins.
    J Virol. 1984 Jun;50(3):922-8 PMID: 6328023
  2. Rapid evolution of RNA genomes.
    Science. 1982 Mar 26;215(4540):1577-85 PMID: 7041255
  3. Complete nucleotide sequences of all three poliovirus serotype genomes. Implication for genetic relationship, gene function and antigenic determinants.
    J Mol Biol. 1984 Apr 25;174(4):561-85 PMID: 6202874
  4. Recombinants of Mahoney and Sabin strain poliovirus type 1: analysis of in vitro phenotypic markers and evidence that resistance to guanidine maps in the nonstructural proteins.
    Virology. 1984 Aug;137(1):74-85 PMID: 6089422
  5. Association of the polioviral RNA polymerase complex with phospholipid membranes.
    J Virol. 1976 Aug;19(2):457-66 PMID: 183011
  6. The complete nucleotide sequence of a common cold virus: human rhinovirus 14.
    Nucleic Acids Res. 1984 Oct 25;12(20):7859-75 PMID: 6093056
  7. SPECIFIC INHIBITION OF REPLICATION OF ANIMAL VIRUSES.
    Science. 1963 Oct 4;142(3588):24-33 PMID: 14057349
  8. Morphogenesis of poliovirus. I. Association of the viral RNA with coat protein.
    J Mol Biol. 1968 Apr 28;33(2):369-78 PMID: 4302632
  9. Systematic nomenclature of picornavirus proteins.
    J Virol. 1984 Jun;50(3):957-9 PMID: 6726891
  10. Antiviral effect of guanidine.
    Science. 1961 Aug 25;134(3478):558-9 PMID: 13741634
  11. The role of cytoplasmic membranes in poliovirus biosynthesis.
    Virology. 1970 Sep;42(1):100-11 PMID: 4318974
  12. Genetic recombination with poliovirus type 1. Studies of crosses between a normal horse serum-resistant mutant and several guanidine-resistant mutants of the same strain.
    Virology. 1963 May;20:107-19 PMID: 13929034
  13. Guanidine inhibits tobacco mosaic virus RNA synthesis at two stages.
    Intervirology. 1975-1976;6(2):83-9 PMID: 1235830
  14. Antigenic variation and resistance to neutralization in poliovirus type 1.
    Science. 1985 Sep 13;229(4718):1090-3 PMID: 2412292
  15. Human rhinovirus 2: complete nucleotide sequence and proteolytic processing signals in the capsid protein region.
    Nucleic Acids Res. 1985 Mar 25;13(6):2111-26 PMID: 2987843
  16. A genetic recombination map of foot-and-mouth disease virus.
    J Gen Virol. 1975 Jun;27(3):355-67 PMID: 167118
  17. Guanidine inhibition of poliovirus: a dependence of viral RNA synthesis on the configuration of structural protein.
    Virology. 1970 Mar;40(3):480-93 PMID: 4314503
  18. Basis for variable response of arboviruses to guanidine treatment.
    J Virol. 1970 Nov;6(5):628-36 PMID: 5530010
  19. Complete nucleotide sequence of the attenuated poliovirus Sabin 1 strain genome.
    Proc Natl Acad Sci U S A. 1982 Oct;79(19):5793-7 PMID: 6310545
  20. Mechanism of infection by Epstein-Barr virus. II. Comparison of viral DNA from HR-1 and superinfected Raji cells by restriction enzymes.
    Virology. 1977 Aug;81(1):17-24 PMID: 196398
  21. Effects on host cell metabolism following synchronous infection with poliovirus.
    Virology. 1965 Dec;27(4):614-20 PMID: 4159191
  22. Nucleotide and amino acid sequence coding for polypeptides of foot-and-mouth disease virus type A12.
    J Virol. 1985 Jun;54(3):651-60 PMID: 2987518
  23. Initiation of polyribosome formation in poliovirus-infected HeLa cells.
    J Mol Biol. 1970 Feb 14;47(3):275-91 PMID: 4313849
  24. Dynamics of poliovirus-specific RNA synthesis and the effects of inhibitors of virus replication.
    Virology. 1970 Mar;40(3):634-42 PMID: 4314509
  25. Defective interfering particles of poliovirus: mapping of the deletion and evidence that the deletions in the genomes of DI(1), (2) and (3) are located in the same region.
    J Mol Biol. 1979 Feb 25;128(2):179-96 PMID: 219204
  26. Primary structure, gene organization and polypeptide expression of poliovirus RNA.
    Nature. 1981 Jun 18;291(5816):547-53 PMID: 6264310
  27. Studies of the inhibitory action of guanidine on poliovirus multiplication in cell cultures.
    Virology. 1961 Sep;15:65-74 PMID: 13696674
  28. Inhibition of tobacco necrosis virus by guanidine carbonate.
    Virology. 1968 Oct;36(2):305-8 PMID: 5684125
  29. Involvement of viral procapsid in the RNA synthesis and maturation of poliovirus.
    Virology. 1977 Oct 15;82(2):299-307 PMID: 199991
  30. Analysis of virus replication in ageing human fibroblast cultures.
    Nature. 1973 Oct 12;245(5424):316-9 PMID: 4357305
  31. Similarity in gene organization and homology between proteins of animal picornaviruses and a plant comovirus suggest common ancestry of these virus families.
    Nucleic Acids Res. 1984 Sep 25;12(18):7251-67 PMID: 6384934
  32. Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome.
    Proc Natl Acad Sci U S A. 1981 Aug;78(8):4887-91 PMID: 6272282
  33. Association of poliovirus proteins with the endoplasmic reticulum.
    J Virol. 1984 Dec;52(3):777-83 PMID: 6092710
  34. Membrane fractions active in poliovirus RNA replication contain VPg precursor polypeptides.
    Virology. 1983 Jul 15;128(1):33-47 PMID: 6308897
  35. Guanidine conditioned infectivity of ribonucleic acid extracted from a strain of guanidine-dependent polio-1-virus.
    Nature. 1963 Jan 19;197:315 PMID: 13931211
  36. Nucleotide sequence and genome organization of foot-and-mouth disease virus.
    Nucleic Acids Res. 1984 Aug 24;12(16):6587-601 PMID: 6089122
  37. Guanidine-resistant mutants of aphthovirus induce the synthesis of an altered nonstructural polypeptide, P34.
    J Virol. 1982 May;42(2):389-94 PMID: 6283162
  38. In vitro inhibition of infectivity of polio viruses by guanidine.
    Nature. 1962 Jan 6;193:97-8 PMID: 14466152
  39. Intertypic recombination in poliovirus: genetic and biochemical studies.
    Virology. 1983 Jan 15;124(1):121-32 PMID: 6297149
  40. Action of guanidine on the replication of poliovirus RNA.
    Virology. 1968 Jul;35(3):408-17 PMID: 4298649
  41. INHIBITION OF MULTIPLICATION OF FOOT-AND-MOUTH DISEASE VIRUS BY GUANIDINE HYDROCHLORIDE.
    Nature. 1964 Dec 5;204:1012-3 PMID: 14248709
  42. The use of inhibitors in the study of picornavirus genetics.
    Prog Med Virol. 1972;14:123-99 PMID: 4555915
  43. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
    Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5 PMID: 6575390
  44. An extended genetic recombination map for foot-and-mouth diseases virus.
    J Gen Virol. 1977 Jun;35(3):555-65 PMID: 196035
  45. Homologous sequences in non-structural proteins from cowpea mosaic virus and picornaviruses.
    EMBO J. 1984 Apr;3(4):855-61 PMID: 16453518
  46. COXSACKIE A9 VIRUS: MUTATION FROM DRUG DEPENDENCE TO DRUG INDEPENDENCE.
    Science. 1965 Apr 2;148(3666):97-8 PMID: 14258734
  47. Defective interfering particles of poliovirus. II. Nature of the defect.
    J Mol Biol. 1973 May 25;76(3):325-43 PMID: 4354477
  48. Inhibition of poliovirus polymerase by guanidine in vitro.
    J Virol. 1982 Jan;41(1):313-8 PMID: 6283124
  49. The complete nucleotide sequence of the RNA coding for the primary translation product of foot and mouth disease virus.
    Nucleic Acids Res. 1984 Mar 12;12(5):2461-72 PMID: 6324120
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.57.2.638-646.1986
Published
1986-02-00
Pages
638-46
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC252779
Subset
IM
Grants
NIAID NIH HHS · AI-06920 · United States
NIAID NIH HHS · AI-15122 · United States
NCI NIH HHS · CA-28146 · United States
External Links
PubMed source DOI: 10.1128/JVI.57.2.638-646.1986 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2