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PMID: 6328023 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Guanidine-resistant poliovirus mutants produce modified 37-kilodalton proteins.

Journal of virology ·Vol. 50 ·No. 3 ·1984-06-00 ·Pages 922-8

Anderson-Sillman K, Bartal S, Tershak DR

Abstract

Eighteen spontaneous, guanidine-resistant mutants of poliovirus were obtained by plaque selection. Isoelectric focusing demonstrated charge changes in a 37-kilodalton protein, pX, among three of the mutants. The precursor of pX, NCVP5b , also exhibited charge changes among the three mutants. pX of 12 mutants was also examined by peptide mapping with Staphylococcus aureus V8 protease. Nine of the mutants presented modified maps, and seven of these maps were identical. The demonstration of mutational changes in pX in 12 of 18 mutants suggests a role for this protein in determining the guanidine trait of poliovirus and corroborates studies with foot-and-mouth disease virus.

MeSH Terms
Drug Resistance, Microbial Guanidine Guanidines/toxicity HeLa Cells Hot Temperature Humans Molecular Weight Mutation Peptide Fragments/analysis Poliovirus/drug effects,genetics Viral Proteins/genetics,isolation & purification Virion/genetics
Chemicals
Guanidines Peptide Fragments Viral Proteins Guanidine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Anderson-Sillman K
Bartal S
Tershak D R
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29 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.50.3.922-928.1984
Published
1984-06-00
Pages
922-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255754
Subset
IM
Grants
NIAID NIH HHS · AI-16932 · United States
External Links
PubMed source DOI: 10.1128/JVI.50.3.922-928.1984 PMC Full Text View journal details
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