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PMID: 3021340 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The mechanism of RNA recombination in poliovirus.

Cell ·Vol. 47 ·No. 3 ·1986-11-07 ·Pages 433-43

Kirkegaard K, Baltimore D

Abstract

We have investigated RNA recombination among poliovirus genomes by analyzing both intratypic and intertypic recombinant crosses involving the same defined genetic markers. Sequence analysis of the recombinant junctions of 13 nonsibling intertypic recombinants showed that intertypic RNA recombination is not site-specific, nor does it require extensive homology between the recombining parents at the crossover site. To discriminate between breaking-rejoining and copy choice mechanisms of RNA recombination, we have inhibited the replication of the recombining parents independently and found opposite effects on the frequency of genetic recombination in intratypic crosses. The results strongly support a copy choice mechanism for RNA recombination, in which the viral RNA polymerase switches templates during negative strand synthesis.

MeSH Terms
Base Sequence Chromosome Mapping Crosses, Genetic Cytopathogenic Effect, Viral DNA-Directed RNA Polymerases/metabolism Genetic Markers Mutation Poliovirus/genetics RNA, Viral/metabolism Recombination, Genetic Structure-Activity Relationship
Chemicals
Genetic Markers RNA, Viral DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kirkegaard K
Baltimore D
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45 references, click to expand
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
DOI
10.1016/0092-8674(86)90600-8
Published
1986-11-07
Pages
433-43
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC7133339
Subset
IM
Grants
NIAID NIH HHS · AI22346 · United States
Databases
GENBANK
M14761
External Links
PubMed source DOI: 10.1016/0092-8674(86)90600-8 PMC Full Text View journal details
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