Recombination in RNA.

Cell ·Vol. 29 ·No. 3 ·1982-07-00 ·Pages 921-8

King AM, McCahon D, Slade WR, Newman JW

Abstract

The aphthovirus genome consists of a single molecule of single-stranded RNA that encodes all the virus-induced proteins. We isolated recombinant aphthoviruses from cells simultaneously infected with temperature-sensitive mutants of two different subtype strains. Analysis of the proteins induced by 16 independently generated recombinants revealed two types of protein pattern, which were consistent with single genetic crossovers on the 5' side and 3' side, respectively, of the central P34-coding region. Recombinants invariably inherited all four coat proteins from the same parent, and novel recombinant proteins were not observed. RNAase T1 fingerprints of virus RNA, prepared from representatives of each recombinant type, confirmed the approximate crossover sites that had been deduced from the inheritance of proteins. These fingerprints provide molecular evidence of recombination at the level of RNA and demonstrate the potential of RNA recombination for producing genetic diversity among picornaviruses.

MeSH Terms

Aphthovirus/genetics Mutation Oligoribonucleotides/analysis RNA, Viral/genetics Recombination, Genetic Viral Proteins/genetics

Chemicals

Oligoribonucleotides RNA, Viral Viral Proteins

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

King A M

McCahon D

Slade W R

Newman J W

References (25)

25 references, click to expand

The genetics of aphthovirus. Brief review.
Arch Virol. 1981;69(1):1-23 PMID: 6271095
Biochemical identification of viruses causing the 1981 outbreaks of foot and mouth disease in the UK.
Nature. 1981 Oct 8;293(5832):479-80 PMID: 6273731
Location of the initiation site for protein synthesis on foot-and-mouth disease virus RNA by in vitro translation of defined fragments of the RNA.
J Virol. 1980 Jan;33(1):59-68 PMID: 6245254
A sensitive method for the detection and isolation of recombinants of foot-and-mouth disease virus.
J Gen Virol. 1981 Apr;53(Pt 2):333-42 PMID: 6267181
High resolution two-dimensional electrophoresis of proteins.
J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
Genetic recombination with Newcastle disease virus, polioviruses, and influenza.
Cold Spring Harb Symp Quant Biol. 1962;27:303-9 PMID: 13954778
18S defective interfering RNA of Semliki Forest virus contains a triplicated linear repeat.
Proc Natl Acad Sci U S A. 1981 Sep;78(9):5353-7 PMID: 6946476
Mouse hepatitis virus A59: mRNA structure and genetic localization of the sequence divergence from hepatotropic strain MHV-3.
J Virol. 1981 Sep;39(3):823-34 PMID: 6169842
A study of the level of nucleotide sequence conservation between the RNAs of two types serotypes of foot-and-mouth disease virus.
J Gen Virol. 1980 Oct;50(2):403-18 PMID: 6257826
The origins of defective interfering particles of the negative-strand RNA viruses.
Cell. 1981 Oct;26(2 Pt 2):145-54 PMID: 7037195
Guanidine-resistant mutants of aphthovirus induce the synthesis of an altered nonstructural polypeptide, P34.
J Virol. 1982 May;42(2):389-94 PMID: 6283162
EVIDENCE OF GENETIC RECOMBINATION IN FOOT-AND-MOUTH DISEASE VIRUS.
Virology. 1965 Jan;25:48-54 PMID: 14277084
On the origin of the human influenza virus subtypes H2N2 and H3N2.
Virology. 1978 Jun 1;87(1):13-20 PMID: 664248
Poliovirus polyuridylic acid polymerase and RNA replicase have the same viral polypeptide.
J Virol. 1979 Jan;29(1):352-60 PMID: 219230
Temperature-sensitive mutants of foot-and-mouth disease virus with altered structural polypeptides. II. Comparison of recombination and biochemical maps.
J Virol. 1980 Apr;34(1):67-72 PMID: 6246263
Genetic recombination with poliovirus type 1. Studies of crosses between a normal horse serum-resistant mutant and several guanidine-resistant mutants of the same strain.
Virology. 1963 May;20:107-19 PMID: 13929034
Biochemical evidence of recombination within the unsegmented RNA genome of aphthovirus.
J Virol. 1982 Jan;41(1):66-77 PMID: 6283129
Temperature-sensitive RNA polymerase mutants of a picornavirus.
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4448-52 PMID: 6270678
Analyses of the genomes of bluetongue viruses recovered in the United States. I. Oligonucleotide fingerprint studies that indicate the existence of naturally occurring reassortant BTV isolates.
Virology. 1981 Oct 15;114(1):210-7 PMID: 6269284
An extended genetic recombination map for foot-and-mouth diseases virus.
J Gen Virol. 1977 Jun;35(3):555-65 PMID: 196035
The replication of picornaviruses.
J Gen Virol. 1979 Oct;45(1):1-13 PMID: 392052
A genetic recombination map of foot-and-mouth disease virus.
J Gen Virol. 1975 Jun;27(3):355-67 PMID: 167118
Temperature-sensitive mutants of foot-and-mouth disease virus with altered structural polypeptides. I. Identification by electrofocusing.
J Virol. 1980 Apr;34(1):59-66 PMID: 6246262
A genetic map of poliovirus temperature-sensitive mutants.
Virology. 1968 Aug;35(4):584-96 PMID: 4300363
Nucleotide sequences of influenza virus segments 1 and 3 reveal mosaic structure of a small viral RNA segment.
Cell. 1982 Feb;28(2):303-13 PMID: 7060132

Article Info

Journal

Cell

Abbr.

Cell

ISSN

0092-8674

DOI

10.1016/0092-8674(82)90454-8

Published

1982-07-00

Pages

921-8

Language

English

Region

United States

NLM ID

0413066

PMCID

PMC7133258

Subset

External Links

PubMed source DOI: 10.1016/0092-8674(82)90454-8 PMC Full Text View journal details

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Recombination in RNA.

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