Home LiteratureArticle Details
PMID: 3015788 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of three HLA-DR beta genes isolated from an HLA-DR 3/4 insulin-dependent diabetic patient.

Immunogenetics ·Vol. 24 ·No. 1 ·1986-00-00 ·Pages 41-6

Owerbach D, Rich C, Taneja K

Abstract

Three HLA-DR beta genes were isolated from a Swedish HLA-DR3/4 insulin-dependent diabetes mellitus (IDDM) patient and characterized by restriction endonuclease mapping and nucleotide sequence analysis. Two out of the three DNA sequences differed from those of published DR beta-chain sequences. A DR beta-gene probe prepared from exon 4 and flanking sequences was used in a Southern blot analysis of blood donors' DNA and DNA from HLA-DR3/4 IDDM patients and HLA-DR-matched healthy control subjects. This probe differentiated HLA-DR3/4 IDDM patients and HLA-DR-matched controls in the Scandinavian population but not in the North American Caucasoid population.

MeSH Terms
Amino Acid Sequence Base Sequence Chromosome Mapping DNA Restriction Enzymes Diabetes Mellitus, Type 1/genetics,immunology Genes HLA-DR Antigens Histocompatibility Antigens Class II/genetics Humans Polymorphism, Genetic
Chemicals
HLA-DR Antigens Histocompatibility Antigens Class II DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Owerbach D
Rich C
Taneja K
References (18)
18 references, click to expand
  1. Complete sequence of an HLA-dR beta chain deduced from a cDNA clone and identification of multiple non-allelic DR beta chain genes.
    EMBO J. 1983;2(3):389-94 PMID: 11894954
  2. Cloning and sequence analysis of the human major histocompatibility complex gene DC-3 beta.
    Proc Natl Acad Sci U S A. 1984 Aug;81(16):5199-203 PMID: 6206493
  3. Characterization of an HLA DR beta pseudogene.
    Proc Natl Acad Sci U S A. 1985 Mar;82(5):1475-9 PMID: 3856276
  4. Both alpha and beta chains of HLA-DC class II histocompatibility antigens display extensive polymorphism in their amino-terminal domains.
    EMBO J. 1984 Feb;3(2):447-52 PMID: 6585297
  5. HLA-D region beta-chain DNA endonuclease fragments differ between HLA-DR identical healthy and insulin-dependent diabetic individuals.
    Nature. 1983 Jun 30;303(5920):815-7 PMID: 6306468
  6. Mutations and selection in the generation of class II histocompatibility antigen polymorphism.
    EMBO J. 1984 Jul;3(7):1655-61 PMID: 6589154
  7. The HLA class II genes and products: the HLA-D region.
    Immunol Today. 1985 Mar;6(3):89-94 PMID: 25291537
  8. Isolation of a cDNA clone coding for an SB beta-chain.
    Nature. 1984 May 3-9;309(5963):76-8 PMID: 6325928
  9. HLA-DR typing in identical twins with insulin-dependent diabetes: difference between concordant and discordant pairs.
    Br Med J (Clin Res Ed). 1983 Jan 22;286(6361):253-5 PMID: 6402059
  10. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  11. Exon-intron organization and complete nucleotide sequence of a human major histocompatibility antigen DC beta gene.
    Proc Natl Acad Sci U S A. 1983 Dec;80(23):7313-7 PMID: 6316358
  12. N-terminal amino acid sequences of the alpha and beta chains of HLA-DR1 and HLA-DR2 antigens.
    Biochemistry. 1983 Jan 4;22(1):185-8 PMID: 6600932
  13. HLA-D and -DR antigens in genetic analysis of insulin dependent diabetes mellitus.
    Diabetologia. 1981 Aug;21(2):108-15 PMID: 6167481
  14. A convenient and adaptable package of computer programs for DNA and protein sequence management, analysis and homology determination.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):643-55 PMID: 6320100
  15. Complete amino acid sequence of an HLA-DR antigen-like beta chain as predicted from the nucleotide sequence: similarities with immunoglobulins and HLA-A, -B, and -C antigens.
    Proc Natl Acad Sci U S A. 1982 Jun;79(12):3687-91 PMID: 6954511
  16. Can future type I diabetes be predicted? A study in families of affected children.
    Diabetes. 1982 Oct;31(10):862-6 PMID: 7152125
  17. Complete nucleotide sequence of a functional HLA-DP beta gene and the region between the DP beta 1 and DP alpha 1 genes: comparison of the 5' ends of HLA class II genes.
    Nucleic Acids Res. 1985 Mar 11;13(5):1607-21 PMID: 2987832
  18. DNA sequence and characterization of human class II major histocompatibility complex beta chains from the DR1 haplotype.
    Proc Natl Acad Sci U S A. 1985 May;82(10):3405-9 PMID: 3858829
Article Info
Journal
Immunogenetics
Abbr.
Immunogenetics
ISSN
0093-7711
DOI
10.1007/BF00372296
Published
1986-00-00
Pages
41-6
Language
English
Region
United States
NLM ID
0420404
Subset
IM
Databases
GENBANK
M15372, M15373, M15374, M15375, M15376, M15377, M15838, M15839
External Links
PubMed source DOI: 10.1007/BF00372296 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2